Almirón I, Chemes H
Laboratory of Testicular Physiology and Pathology, Buenos Aires Children's Hospital, Buenos, Argentina.
Int J Androl. 1988 Jun;11(3):235-46. doi: 10.1111/j.1365-2605.1988.tb00998.x.
By using high doses of testosterone propionate (TP) endogenous FSH was lowered to non-detectable levels in immature rats of different ages. Combined administration of TP and human menopausal gonadotrophin (hMG) or purified human FSH (hFSH) restored circulating FSH to normal or supranormal levels. This experimental model was used to investigate the influences of hormones on the proliferation of Sertoli and germ cells. Mitoses in seminiferous tubules were counted after being blocked by administration of colchicine. The mitotic index so determined showed a reduction with FSH withdrawal and a significant increase when the hormone levels were restored. Testicular DNA content was determined in testicular homogenates and showed similar changes. Autoradiographs were prepared and 3H-thymidine incorporation into germ and Sertoli cells was quantified. It was found that hFSH induced a significant increase in the labelling indices of gonocytes, type A-spermatogonia and Sertoli cells. All of these changes were effective in rats younger than 10 days but no modifications in any of the parameters under study were observed after 20 days. It is concluded that FSH exerts a stimulatory effect on the proliferation of spermatogonia, Sertoli cells and testicular DNA content during the first 10 days of life.
通过使用高剂量丙酸睾酮（TP），不同年龄的未成熟大鼠体内的内源性促卵泡激素（FSH）降至检测不到的水平。联合给予TP和人绝经期促性腺激素（hMG）或纯化的人促卵泡激素（hFSH）可使循环中的FSH恢复到正常或超正常水平。该实验模型用于研究激素对支持细胞和生殖细胞增殖的影响。在给予秋水仙碱阻断后，对生精小管中的有丝分裂进行计数。如此确定的有丝分裂指数显示，FSH撤除后降低，而激素水平恢复时则显著增加。测定睾丸匀浆中的睾丸DNA含量，结果显示出类似变化。制备放射自显影片并对3H-胸腺嘧啶核苷掺入生殖细胞和支持细胞的情况进行定量分析。结果发现，hFSH可使生殖母细胞、A型精原细胞和支持细胞的标记指数显著增加。所有这些变化在10日龄以下的大鼠中有效，但在20日龄后未观察到所研究的任何参数有改变。结论是，FSH在出生后的前10天对精原细胞、支持细胞的增殖以及睾丸DNA含量发挥刺激作用。
