邻苯二甲酸二甲酯（DMP）对C57雌性小鼠血清性激素水平及细胞凋亡的影响
Effects of Dimethyl Phthalate (DMP) on Serum Sex Hormone Levels and Apoptosis in C57 Female Mice.
作者信息
Mei Yue, Rongshuang Ma, Ruizhi Zhang, Hongyuan Huang, Qiyue Tan, Shuhua Zhao
机构信息
Jilin University School of Public Health, Changchun, China.
出版信息
Int J Endocrinol Metab. 2019 Apr 22;17(2):e82882. doi: 10.5812/ijem.82882. eCollection 2019 Apr.
Abstract
BACKGROUND
The effects of dimethyl phthalate (DMP) on the reproductive system of mammal females are unclear because no studies have been conducted on this topic.
METHODS
In this study, 40 C57 female mice were used as experimental subjects and evenly divided into 8 groups, which were fed with mixed DMP (0, 0.5, 1, and 2 g/kg bw/day) and corn oil. After 20 days and 40 days of gavage, the mice were weighed and their individual ovary organ coefficients measured.
RESULTS
Changes were discovered on progesterone, estradiol, follicle-stimulating hormone and luteinizing hormone in mouse serum, and on the apoptosis rate of ovarian granulosa cells.
CONCLUSIONS
Prolonged exposure to DMP led to decreased secretion of FSH hormones and increased secretion of E2 and LH hormones. Furthermore, DMP interfered with the pituitary-ovary axis and increased the apoptosis rate of ovarian granulosa cells. Therefore, prolonged exposure to DMP is likely to have negative effects on reproduction and development.
摘要
背景
由于尚未开展关于邻苯二甲酸二甲酯（DMP）对哺乳动物雌性生殖系统影响的研究，其影响尚不清楚。
方法
本研究以40只C57雌性小鼠为实验对象，将其平均分为8组，分别用混合DMP（0、0.5、1和2克/千克体重/天）和玉米油进行灌胃。在灌胃20天和40天后，对小鼠进行称重并测量其个体卵巢脏器系数。
结果
发现小鼠血清中孕酮、雌二醇、促卵泡激素和促黄体生成素发生了变化，同时卵巢颗粒细胞凋亡率也发生了变化。
结论
长期接触DMP导致促卵泡激素分泌减少，雌二醇和促黄体生成素分泌增加。此外，DMP干扰了垂体-卵巢轴，增加了卵巢颗粒细胞凋亡率。因此，长期接触DMP可能对生殖和发育产生负面影响。
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Effects of Dimethyl Phthalate (DMP) on Serum Sex Hormone Levels and Apoptosis in C57 Female Mice.邻苯二甲酸二甲酯（DMP）对C57雌性小鼠血清性激素水平及细胞凋亡的影响
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