同型半胱氨酸对正常和肥胖小鼠脂肪组织中 CD36、PPARγ 和 C/EBPα 表达的影响。
The effect of homocysteine on the expression of CD36, PPARγ, and C/EBPα in adipose tissue of normal and obese mice.
机构信息
Program of Medical Laboratory Techniques, Vocational School of Health Sciences, Karadeniz Technical University, Trabzon, Turkey.
Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey.
出版信息
Arch Physiol Biochem. 2021 Oct;127(5):437-444. doi: 10.1080/13813455.2019.1648517. Epub 2019 Aug 2.
The purpose of this study was to investigate the effect of homocysteine (Hcy) on CD36, PPARγ, and C/EBPα gene and protein expression in adipose tissue obtained from normal and high-calorie diet obesity models. CD36, PPARγ, and C/EBPα gene expression and protein levels in adipose tissue specimens were determined using the RT-PCR and ELISA methods, respectively. Significantly increased CD36 gene expression was observed in adipose tissue from obese mice, while Hcy significantly reduced CD36 gene expression in adipose tissue from normal and obese mice. PPARγ and C/EBPα gene expression levels decreased significantly in all groups compared to the normal group. In addition, levels of both PPARγ and C/EBPα gene expression were lower with Hcy supplementation compared to their own controls. In conclusion, Hcy's reduction of CD36 gene expression in adipose tissue may be one probable factor in hyperhomocysteinemia representing an independent risk factor for cardiovascular diseases.
本研究旨在探讨同型半胱氨酸（Hcy）对正常和高热量饮食肥胖模型脂肪组织中 CD36、PPARγ 和 C/EBPα 基因和蛋白表达的影响。采用 RT-PCR 和 ELISA 法分别测定脂肪组织标本中 CD36 基因表达和蛋白水平。肥胖小鼠脂肪组织中 CD36 基因表达明显增加，而 Hcy 可明显降低正常和肥胖小鼠脂肪组织中 CD36 基因表达。与正常组相比，各组 PPARγ 和 C/EBPα 基因表达水平均显著降低。此外，与各自对照相比，Hcy 补充后 PPARγ 和 C/EBPα 基因表达水平均较低。总之，Hcy 降低脂肪组织中 CD36 基因表达可能是高同型半胱氨酸血症代表心血管疾病独立危险因素的一个可能因素。
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