Stepniak Jan, Lewinski Andrzej, Karbownik-Lewinska Malgorzata
Department of Oncological Endocrinology, Medical University of Lodz, Poland.
Department of Endocrinology and Metabolic Diseases, Medical University of Lodz, Poland.
Drug Chem Toxicol. 2021 Nov;44(6):655-660. doi: 10.1080/01480545.2019.1643878. Epub 2019 Aug 2.
It has long been observed that thyroid diseases are more prevalent in women than in men. However, there are limited experimental data demonstrating mechanisms by which sex differences in thyroid diseases may occur and exact molecular mechanisms involved are still far from clear. The aim of the study was to evaluate if there are sex differences concerning oxidative damage to membrane lipids in thyroid homogenates in response to Fenton reaction substrates, i.e., Fe and/or HO, and, additionally, in response to potentially protective agent, i.e., melatonin. Homogenates of male or female thyroids collected from adult swine () at slaughter were incubated in the presence of HO and/or Fe without or with addition of melatonin. Malondialdehyde + 4-hydroxyalkenals concentration (LPO index) was measured spectrophotometrically. Neither HO nor Fe, when used separately, did affect the level of lipid peroxidation in both male and female porcine thyroid homogenates. When HO (0.5 mM) was used together with different concentrations of Fe, the level of lipid peroxidation increased significantly in both male and female porcine thyroid homogenates, with clear Fe concentration-dependent stimulatory effect, but without differences between sexes. No sex-specific differences was found concerning oxidative damage to membrane lipids in porcine thyroid in response to Fenton reaction substrates and/or to melatonin. The lack of expected differences may be due to potentially lower sensitivity of membrane lipids comparing to other biological macromolecules to pro-/antioxidative agents in the thyroid. However, further studies should be performed to explain the discussed issue.
长期以来人们一直观察到，甲状腺疾病在女性中比在男性中更为普遍。然而，证明甲状腺疾病中性别差异可能出现的机制的实验数据有限，所涉及的确切分子机制仍远未明确。本研究的目的是评估在甲状腺匀浆中，针对芬顿反应底物（即铁和/或羟基自由基）以及另外针对潜在保护剂褪黑素，膜脂质的氧化损伤是否存在性别差异。从成年猪（）屠宰时采集的雄性或雌性甲状腺匀浆，在有或没有添加褪黑素的情况下，于羟基自由基和/或铁存在的条件下进行孵育。通过分光光度法测量丙二醛 + 4 - 羟基烯醛浓度（脂质过氧化指数）。单独使用羟基自由基或铁时，均未影响雄性和雌性猪甲状腺匀浆中的脂质过氧化水平。当羟基自由基（0.5 mM）与不同浓度的铁一起使用时，雄性和雌性猪甲状腺匀浆中的脂质过氧化水平均显著升高，呈现明显的铁浓度依赖性刺激效应，但无性别差异。在猪甲状腺中，针对芬顿反应底物和/或褪黑素，膜脂质的氧化损伤未发现性别特异性差异。缺乏预期差异可能是由于与甲状腺中其他生物大分子相比，膜脂质对促氧化/抗氧化剂的敏感性可能较低。然而，应进行进一步研究以解释所讨论的问题。
