Drahorád J, Cechová D, Tesarík J
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
J Reprod Fertil. 1988 Jul;83(2):599-603. doi: 10.1530/jrf.0.0830599.
Components of human follicular fluid were separated on Sepharose 6B columns and the effects of different fractions on the conversion of pig proacrosin to acrosin were examined. A high-molecular-weight fraction (Mr greater than 3,000,000) of follicular fluid was a potent stimulator of this reaction. The proacrosin converting activity was absent in the corresponding fraction of blood serum. The acceleration of proacrosin activation was dependent on the concentration of material with proacrosin converting activity. The results indicate that human follicular fluid contains a high-molecular-weight component of local origin which is capable of accelerating proacrosin in a dose-dependent manner.
将人卵泡液的成分在琼脂糖6B柱上进行分离，并检测不同组分对猪前顶体蛋白酶转化为顶体蛋白酶的影响。卵泡液的一种高分子量组分（分子量大于3,000,000）是该反应的有效刺激物。血清的相应组分中不存在前顶体蛋白酶转化活性。前顶体蛋白酶激活的加速取决于具有前顶体蛋白酶转化活性的物质的浓度。结果表明，人卵泡液含有一种局部来源的高分子量成分，其能够以剂量依赖的方式加速前顶体蛋白酶的转化。
