Stambaugh R, Mastroianni L
J Reprod Fertil. 1980 Jul;59(2):479-84. doi: 10.1530/jrf.0.0590479.
Pronase-resistant low molecular weight stimulators for the activation of proacrosin to acrosin were found in rhesus monkey oviduct fluid collected before, during and after ovulation, but the presence of high concentrations of acrosin inhibitors before and after ovulation partly masked the stimulation in unfractionated fluid. This low molecular weight fraction of oviduct fluid had no detectable esterase or amidase activity by itself, and the stimulating factors were sensitive to digestion by hyaluronidase and chondroitin ABC lyase and were presumed to be glycosaminoglycans. Heparin and hyaluronic acid had similar effects. The presence of soluble glycosaminoglycans at the site of fertilization suggests that they may have a role in capacitation and fertilization.
在排卵前、排卵期间和排卵后收集的恒河猴输卵管液中发现了对链霉蛋白酶有抗性的低分子量刺激物，可将前顶体蛋白酶激活为顶体蛋白酶，但排卵前后高浓度顶体蛋白酶抑制剂的存在部分掩盖了未分级液体中的刺激作用。输卵管液的这种低分子量部分本身没有可检测到的酯酶或酰胺酶活性，刺激因子对透明质酸酶和软骨素ABC裂合酶的消化敏感，推测为糖胺聚糖。肝素和透明质酸有类似作用。受精部位可溶性糖胺聚糖的存在表明它们可能在获能和受精中起作用。
