Davis D S, Heck F C, Williams J D, Simpson T R, Adams L G
Department of Veterinary Pathology, College of Veterinary Medicine, Texas A&M University, College Station 77843.
J Wildl Dis. 1988 Jul;24(3):533-7. doi: 10.7589/0090-3558-24.3.533.
In four separate trials, 10 coyotes (Canis latrans) which had been individually fed mascerated bovine placental tissue experimentally inoculated with Brucella abortus strain 2308 were placed in a 1 ha isolation area with six parturient, non-B. abortus vaccinated, Brucella spp. seronegative Hereford heifers. During the second trial, three of the heifers became Brucella spp. seroreactive (as determined by the card, standard agglutination tube, rivanol, complement fixation, and enzyme labeled immunosorbent assay tests) 14 days after exposure to the B. abortus infected coyotes. Five of 10 coyotes in the second trial seroconverted to Brucella spp. positive by day 14 postexposure (PE) and by day 27 PE seven of the 10 coyotes were Brucella spp. reactive. The three Brucella spp. seroreactive heifers subsequently aborted on days 35, 64 and 66 PE and B. abortus strain 2308 was isolated from vaginal swabs, milk, and placental and fetal tissues. There was no serologic or bacteriologic evidence of transmission from B. abortus infected coyotes to the susceptible cattle recorded in the other three trials.
在四项独立试验中，将10只分别喂食了经实验接种流产布鲁氏菌2308菌株的牛胎盘组织匀浆的郊狼（犬属）放置在一个1公顷的隔离区域内，与6头临产的、未接种流产布鲁氏菌疫苗、布鲁氏菌属血清学阴性的赫里福德小母牛在一起。在第二项试验中，3头小母牛在接触感染流产布鲁氏菌的郊狼14天后，布鲁氏菌属血清反应呈阳性（通过卡片、标准凝集试管、利凡诺、补体结合和酶联免疫吸附试验检测确定）。在第二项试验中，10只郊狼中有5只在接触后第14天血清转化为布鲁氏菌属阳性，到接触后第27天，10只郊狼中有7只布鲁氏菌属反应呈阳性。这3只布鲁氏菌属血清反应呈阳性的小母牛随后在接触后第35、64和66天流产，并且从阴道拭子、牛奶以及胎盘和胎儿组织中分离出流产布鲁氏菌2308菌株。在其他三项试验中，没有记录到感染流产布鲁氏菌的郊狼向易感牛只传播的血清学或细菌学证据。
