Nicoletti P, Tanya V
Department of Infectious Diseases, College of Veterinary Medicine, University of Florida, Gainesville 32611-0880.
J Am Vet Med Assoc. 1993 Jun 15;202(12):1975-7.
Bacteriologic culture of udder secretions and card, rivanol, and complement fixation tests for detection of brucellosis were performed on samples from 828 cows vaccinated with strain 19 in herds with brucellosis. An ELISA and a particle concentration fluorescence immunoassay were performed on sera from 560 and 569 cows, respectively. A field strain of Brucella abortus or strain 19 was isolated from 278 cows. The ELISA and the card test had high sensitivity, but low specificity. Data suggested that there is no advantage to using primary binding assays rather than the simple buffered antigen agglutination procedures to detect cows infected with a field strain of B abortus.
对来自布鲁氏菌病流行牛群中828头接种19号菌株疫苗的奶牛样本，进行了乳房分泌物细菌学培养以及用于检测布鲁氏菌病的平板凝集试验、鲁米那试验和补体结合试验。分别对560头和569头奶牛的血清进行了ELISA和颗粒浓度荧光免疫测定。从278头奶牛中分离出流产布鲁氏菌野毒株或19号菌株。ELISA和平板凝集试验敏感性高，但特异性低。数据表明，在检测感染流产布鲁氏菌野毒株的奶牛时，使用直接结合试验并不比简单的缓冲抗原凝集程序更具优势。
