Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, Indiana.
Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, Indiana.
Biotechnol Prog. 2019 Nov;35(6):e2889. doi: 10.1002/btpr.2889. Epub 2019 Aug 20.
Millions of Americans contract food poisoning or are affected by microbial pathogens each year. Rapid, sensitive detection of dilute levels of pathogens in foods, produce, water, and biomanufacturing process samples is key to consumer protection; however, current enrichment methods require as much as a full day to enrich viable bacterial pathogens to detectable levels. Our lab previously demonstrated the ability to concentrate and detect dilute levels of pathogens, within 8 hr, from various food matrices using microfiltration in our continuous cell concentration device (i.e., C3D) with one or two filter modules. This short communication describes the design, materials and construction, layout, and operational characteristics of a four filter module multiplexed system based on a four channel device. Benefits are a 2× greater sample capacity than an equivalent duplex system (achieving the same time to result of less than 8 hr from sample preparation to detection), simpler operation, and a footprint enabling operation inside a biosafety cabinet instead of requiring a BSL-2 room. Flow rate variability through four channels fit within an operational envelope of ±3%; flow rates are reproducible from one run to the next thus ensuring relatively simple, concurrent processing of samples.
每年都有数百万美国人感染食源性疾病或受到微生物病原体的影响。快速、灵敏地检测食品、农产品、水和生物制造过程样品中稀释水平的病原体，是保护消费者的关键；然而，目前的富集方法需要长达一整天的时间才能将活菌病原体富集到可检测的水平。我们实验室之前已经证明，使用我们的连续细胞浓缩装置（即 C3D）中的微滤，在一个或两个过滤模块的作用下，能够在 8 小时内浓缩和检测到来自各种食品基质的稀释水平的病原体。本简短交流描述了一种基于四通道设备的四过滤模块多路复用系统的设计、材料和结构、布局和操作特点。其优点是与同等的双工系统相比，样本容量增加了 2 倍（从样品制备到检测的结果不到 8 小时，即可达到相同的时间），操作更简单，占地面积更小，可以在生物安全柜内操作，而不需要 BSL-2 房间。四个通道的流量变化在操作范围的±3%以内；流量是可重复的，从一次运行到下一次运行，从而确保相对简单、同时处理样品。
