Department of School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Department of School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Free Radic Biol Med. 2019 Nov 1;143:47-54. doi: 10.1016/j.freeradbiomed.2019.07.033. Epub 2019 Jul 30.
Alcoholic liver disease (ALD) is a major cause of liver injury worldwide. Oxidative damage is one of the main injuries caused by ALD. The aim of this study was to elucidate the preventive effects of torularhodin, extracted from Sporidiobolus pararoseus, on alcoholic liver injury in mice. The mechanisms involved were investigated using transcriptome analysis. Torularhodin supplementation decreased ethanol-induced aspartate transaminase (ALT), aspartate transaminase (AST) and low density lipoprotein (LDL) levels, and increased high density lipoprotein (HDL) levels in the serum of mice. In liver tissue, treatment with torularhodin increased ethanol-induced superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) levels and decreased tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) levels. Histological analysis showed that torularhodin could alleviate the negative effects of alcohol on the liver. Transcriptomic analysis showed that 806 genes were significantly differentially expressed (506 up-regulated and 300 down-regulated) after torularhodin treatment. These genes were involved in three main Gene Ontology categories (biological process, cellular component, and molecular function) and multiple pathways. Therefore, torularhodin was considered to have potential as a protective agent against ALD.
酒精性肝病 (ALD) 是全球范围内导致肝损伤的主要原因之一。氧化损伤是 ALD 引起的主要损伤之一。本研究旨在阐明从玫瑰红侧耳中提取的 torularhodin 对小鼠酒精性肝损伤的预防作用。通过转录组分析研究了所涉及的机制。Torularhodin 补充剂可降低乙醇诱导的天冬氨酸转氨酶 (ALT)、天冬氨酸转氨酶 (AST) 和低密度脂蛋白 (LDL) 水平，并增加小鼠血清中的高密度脂蛋白 (HDL) 水平。在肝组织中，torularhodin 处理可增加乙醇诱导的超氧化物歧化酶 (SOD) 和谷胱甘肽过氧化物酶 (GSH-Px) 水平，并降低肿瘤坏死因子-α (TNF-α) 和白细胞介素-1β (IL-1β) 水平。组织学分析表明，torularhodin 可以减轻酒精对肝脏的负面影响。转录组分析显示，torularhodin 处理后有 806 个基因显著差异表达（506 个上调，300 个下调）。这些基因参与了三个主要的基因本体类别（生物过程、细胞成分和分子功能）和多个途径。因此，torularhodin 被认为是一种具有潜力的 ALD 保护剂。
