Innovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Genome Biol Evol. 2021 Dec 1;13(12). doi: 10.1093/gbe/evab258.
Sporobolomyces roseus is an important oleaginous red yeast with critical biotechnological applications and has received significant recognition as a valuable source of industrial enzymes, carotenoids, and lipids. To reveal the genetic basis and functional components underlying its biotechnological applications, a high-quality genome assembly is required. Here, we present a novel genome assembly of S. roseus CGMCC 2.4355 using a combination of Illunima and Oxford Nanopore technologies. The genome has an assembly size of 21.4 Mb and consists of 15 scaffolds with an N50 length of 2,126,566 bp and GC content of 49.52%. The assembly is of high integrity, comprising 95.2% complete Benchmarking Universal Single-Copy Orthologs (BUSCOs) as evaluated by a genome completeness assessment. The genome was predicted to contain 8,124 protein-coding genes, 6,890 of which were functionally annotated. We believe that the combination of our analyses and high-quality genome assembly will promote the basic development of S. roseus as an agent for biotechnological applications and make a significant contribution to assess the evolutionary relationship of Sporobolomyces species.
玫瑰掷孢酵母是一种重要的产油红色酵母，具有关键的生物技术应用，已被公认为工业酶、类胡萝卜素和脂质的有价值来源。为了揭示其生物技术应用的遗传基础和功能组成，需要高质量的基因组组装。在这里，我们使用 Illumina 和 Oxford Nanopore 技术的组合，呈现了玫瑰掷孢酵母 CGMCC 2.4355 的新型基因组组装。该基因组的组装大小为 21.4 Mb，由 15 个支架组成，N50 长度为 2,126,566 bp，GC 含量为 49.52%。该组装具有高度完整性，完整性评估表明其包含 95.2%的完整 Benchmarking Universal Single-Copy Orthologs（BUSCOs）。基因组预测包含 8,124 个蛋白质编码基因，其中 6,890 个具有功能注释。我们相信，我们的分析和高质量基因组组装的结合将促进玫瑰掷孢酵母作为生物技术应用的基础发展，并为评估掷孢酵母属物种的进化关系做出重大贡献。
