Department of Zoology, Maharshi Dayanand University, Rohtak, 124001, Haryana, India.
Department of Zoology, Maharshi Dayanand University, Rohtak, 124001, Haryana, India.
Carbohydr Res. 2019 Sep 1;483:107749. doi: 10.1016/j.carres.2019.107749. Epub 2019 Jul 17.
For the construction of galactose biosensor, chitosan was electropolymerised onto the pencil graphite electrode. This chitosan modified pencil graphite electrode acts as good matrix for immobilization of enzyme nanoparticles of galactose oxidase. Development of this nanocomposite was further confirmed by Fourier transform infrared spectroscopy and scanning electron microscopy. The presence of chitosan makes the present galactose biosensor more efficient, reproducible and stable. The sensitivity was reported 7 × 10 mA/mM/cm with linear range from 0.05 to 25 mM and better detection limit of 0.05 mM. When the solution of galactose was spiked with 0.5 mM and 1 mM, the analytical recoveries were found 98.6% and 97.6%. A better storage stability was achieved (90days) when compared to earlier reported biosensors.
为了构建半乳糖生物传感器，将壳聚糖电聚合到铅笔石墨电极上。这种壳聚糖修饰的铅笔石墨电极作为固定化半乳糖氧化酶纳米颗粒的良好基质。傅里叶变换红外光谱和扫描电子显微镜进一步证实了这种纳米复合材料的发展。壳聚糖的存在使目前的半乳糖生物传感器更有效、更可重复和更稳定。报道的灵敏度为 7×10 mA/mM/cm，线性范围从 0.05 到 25 mM，检测限更好，为 0.05 mM。当半乳糖溶液中加入 0.5 mM 和 1 mM 时，分析回收率分别为 98.6%和 97.6%。与之前报道的生物传感器相比，该生物传感器具有更好的储存稳定性（90 天）。
