College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Environ Pollut. 2019 Nov;254(Pt A):112906. doi: 10.1016/j.envpol.2019.07.074. Epub 2019 Jul 20.
Glyphosate is the active ingredient in Roundup, one of the most popular herbicides in the world, and its toxicity has caused increasing concerns. The present study aims to investigate the toxic effects of prenatal exposure to pure glyphosate or Roundup on lipid metabolism in offspring. During gestational days (GDs), ICR mice (from Institute of Cancer Research) were given distilled water, 0.5% glyphosate solution (w/v, 0.5 g/100 ml) or 0.5%-glyphosate Roundup solution orally. The livers and serum samples of the offspring were collected on gestational day 19 (GD19), postnatal day 7 (PND7) and PND21. The results showed a significant decrease in the body weight and obvious hepatic steatosis with excessive lipid droplet formation in offspring. Moreover, the concentrations of lipids such as triglycerides (TGs), total cholesterol (T-CHO), and low-density lipoprotein cholesterols (LDL-C) increased to a significant extent in both the serum and livers. Furthermore, there were significant differences in the expression levels of the genes SREBP1C, SREBP2, Fasn, Hmgcr, Hmgcs and PPARα, which are related to lipid biosynthesis or catabolism in the liver. These results demonstrate that chronic prenatal exposure to glyphosate can result in lipid metabolism disruption in the offspring of mice, as glyphosate exerts a negative influence on the expression of lipogenesis genes.
草甘膦是世界上最受欢迎的除草剂之一“农达”的有效成分，其毒性引起了越来越多的关注。本研究旨在探讨产前暴露于纯草甘膦或农达对后代脂质代谢的毒性作用。在妊娠期（GDs），ICR 小鼠（来自癌症研究所）分别给予蒸馏水、0.5%草甘膦溶液（w/v，0.5g/100ml）或 0.5%-草甘膦农达溶液灌胃。在 GD19、PND7 和 PND21 收集后代的肝脏和血清样本。结果表明，后代体重明显下降，肝内出现明显的脂肪变性和过多的脂滴形成。此外，血清和肝脏中的甘油三酯（TGs）、总胆固醇（T-CHO）和低密度脂蛋白胆固醇（LDL-C）等脂质浓度显著升高。此外，肝脏中与脂质生物合成或分解代谢相关的基因 SREBP1C、SREBP2、Fasn、Hmgcr、Hmgcs 和 PPARα 的表达水平也存在显著差异。这些结果表明，慢性产前暴露于草甘膦可导致小鼠后代脂质代谢紊乱，草甘膦对脂肪生成基因的表达产生负面影响。
