Fujii T
Department of Parasitology, School of Medicine, University of the Ryukyus, Okinawa, Japan.
Parasitol Res. 1988;74(5):476-83. doi: 10.1007/BF00535149.
Following the infection of rats with Angiostrongylus cantonensis, the occurrence and molecular features of circulating antigens (CAs) were analyzed, with special reference to their origin in worms, by SDS-PAGE combined with an immunoblotting technique. Antisera against the CAs were obtained by immunizing rats with sera from rats 35, 91, and 150 days postinfection. The anti-sera, referred to as anti-CA(35), anti-CA(91), and anti-CA(150), respectively, formed one or two precipitin lines when tested in Ouchterlony plates against extracts of digestive organs (DE) and of reproductive organs (RE) from adult female worms. When the anti-CA(35) was used as a blotting antibody under nonreducing conditions, a set of clearly spaced narrow bands with molecular weights (mol. wt.) in the range of 90,000-180,000 daltons developed only in the case of the DE. Besides the antigen(s), additional bands with mol.wt. of 115,000 and 185,000 daltons were revealed in the case of the RE when two other antisera were used. Immunoblot analysis of the immunoprecipitates, derived from anti-CA(150) and sera of infected rats, revealed the occurrence of two types of protein as the major CAs: one had a mol.wt. in the range of 140,000-180,000 daltons and was found in the serum 14 days postinfection, and the other, with a mol.wt. of 185,000 daltons, was found in the serum 35-150 days postinfection. Immunohistochemical studies localized the CAs predominantly in the cytoplasm of both uterine eggs and maturing oocytes.
用广州管圆线虫感染大鼠后，采用SDS - 聚丙烯酰胺凝胶电泳结合免疫印迹技术分析循环抗原（CAs）的出现情况及其分子特征，并特别参考其在虫体中的起源。通过用感染后35天、91天和150天大鼠的血清免疫大鼠获得抗循环抗原血清。这些抗血清分别称为抗CA(35)、抗CA(91)和抗CA(150)，在免疫双扩散板中与成年雌虫消化器官（DE）和生殖器官（RE）提取物进行检测时，形成一条或两条沉淀线。当在非还原条件下将抗CA(35)用作印迹抗体时，仅在消化器官提取物的情况下出现一组间距清晰的窄带，其分子量在90,000 - 180,000道尔顿范围内。除了该抗原外，当使用另外两种抗血清时，在生殖器官提取物的情况下还显示出分子量为115,000和185,000道尔顿的其他条带。对源自抗CA(150)和感染大鼠血清的免疫沉淀物进行免疫印迹分析，发现有两种类型的蛋白质作为主要循环抗原：一种分子量在140,000 - 180,000道尔顿范围内，在感染后14天出现在血清中；另一种分子量为185,000道尔顿，在感染后35 - 150天出现在血清中。免疫组织化学研究表明，循环抗原主要定位于子宫卵和成熟卵母细胞的细胞质中。
