Department of Genetics and Evolution, University of Geneva, Sciences III, 30 Quai Ernest-Ansermet, CH-1211, Geneva-4, Switzerland.
Department of Genetics and Evolution, University of Geneva, Sciences III, 30 Quai Ernest-Ansermet, CH-1211, Geneva-4, Switzerland.
Curr Opin Insect Sci. 2019 Dec;36:33-38. doi: 10.1016/j.cois.2019.06.010. Epub 2019 Jun 29.
Drosophila circadian circuit is one of the best described neural circuits but is complex enough to obscure our understanding of how it actually works. Animals' rhythmic behavior, the seemingly simple outcome of their internal clocks, relies on the interaction of heterogeneous clock neurons that are spread across the brain. Direct observations of their coordinated network interactions can bring us forward in understanding the circuit. The current challenge is to observe activity of each of these neurons over a long span of time - hours to days - in live animals. Here we review the progress in circadian circuit interrogation powered by in vivo calcium imaging.
果蝇的生物钟回路是研究最为透彻的神经回路之一，但它非常复杂，使得我们难以理解其实际工作方式。动物的节律行为是其内部时钟的简单表现，但实际上依赖于散布在大脑中的异构生物钟神经元的相互作用。直接观察它们协调的网络相互作用，可以帮助我们进一步了解该回路。目前的挑战是在活体动物中长时间（数小时到数天）观察这些神经元中的每一个的活动。在这里，我们回顾了利用活体钙成像技术进行生物钟回路检测的进展。
