Postgraduate Program in Biophotonics Applied to Health Sciences and Rehabilitation Sciences, University Nove de Julho (UNINOVE), Rua Vergueiro, 235/249, Liberdade, São Paulo, 01504-001, Brazil.
Undergraduate student in Program in Biophotonics Applied to Health Sciences and Rehabilitation Sciences, University Nove de Julho (UNINOVE), Rua Vergueiro, 235/249 - Liberdade, São Paulo, 01504-001, Brazil.
Photodiagnosis Photodyn Ther. 2019 Dec;28:201-209. doi: 10.1016/j.pdpdt.2019.07.022. Epub 2019 Aug 1.
This study aimed at evaluating the remineralization effect promoted by different bioactive fillers on simulated early caries lesions in enamel (ECLE). Forty sound bovine incisors were used to prepare buccal enamel specimens (6 × 6 × 2 mm). The specimens were divided into two areas (3 × 3 mm²): control (CTR) and experimental (EXP). All the enamel specimens were then submitted to a specific protocol for a period of 48 h to induce simulated caries lesions. Subsequently, the specimens were treated for 7 days (2 min, twice a day) with a slurry pastes containing different bioactive particles (P/L ratio: 1 g/mL). All the specimens were analyzed by laser speckle before and after treatments. The results showed after the first analysis that the ECLE had very low average intensity (back-scattered light). Conversely, after application of the bioactive pastes, higher average intensity was always detected; this was comparable to CTR sound specimens. In conclusion, innovative pastes/gels developed for enamel remineralization should contain bioactive particles that when applied daily on early caries lesions may "boost" the remineralization process to reestablish a sound enamel.
本研究旨在评估不同生物活性填料对模拟早期釉质龋损（ECLE）的再矿化效果。使用 40 颗健康的牛切牙制备颊釉质标本（6×6×2mm）。标本分为两个区域（3×3mm²）：对照组（CTR）和实验组（EXP）。所有釉质标本均采用特定方案处理 48 小时，以诱导模拟龋损。随后，用含有不同生物活性颗粒的糊剂（P/L 比：1g/mL）处理标本 7 天（每天两次，每次 2 分钟）。所有标本在处理前后均采用激光散斑进行分析。结果表明，第一次分析后，ECLE 的平均强度（背散射光）非常低。相反，在应用生物活性糊剂后，总是检测到更高的平均强度；这与对照组的健康标本相当。总之，用于釉质再矿化的创新糊剂/凝胶应含有生物活性颗粒，每天应用于早期龋损，可能会“促进”再矿化过程，以重新建立健康的釉质。
