Shanghai Food Safety and Engineering Technology Research Center, Bor S. Luh Food Safety Research Center, Key Lab of Urban Agriculture (South), School of Agriculture & Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Technical Institute of Physics and Chemistry, CAS, Beijing 100190, China.
Food Chem. 2019 Dec 15;301:125280. doi: 10.1016/j.foodchem.2019.125280. Epub 2019 Jul 29.
Self-assembled nanoparticles using the biopolymers chitosan (CH) and chondroitin sulfate (CS) were developed to improve the biological activity of anthocyanin (ACN). The 86.32 ± 0.15% (w/w) of ACN was incorporated into ACN/CH/CS nanoparticles, with the particle size of 350.1 ± 0.99 nm in diameter (i.d.) and 42.55 ± 0.54 in zeta potential (mV). Morphological study and thermogravimetric analysis suggested that the ACN/CH/CS nanoparticles exhibited heterogeneous morphology and high thermal stability. Significant increases in apoptosis by 12.1% and 35.1% were observed with 0.05 mg/ml ACN and ACN/CH/CS nanoparticles in the HCT-116 cell line, indicating that the nanoparticle system led to significant increase in apoptosis (p < 0.05). Structural changes in mitochondria caused by ACN/CH/CS nanoparticles indicated that the nanoparticles had negative impacts on mitochondria. These results showed that nanoparticles could potentially be used as a carrier system to improve the efficacy of ACN.
采用壳聚糖（CH）和硫酸软骨素（CS）两种生物聚合物自组装纳米颗粒，以提高花色苷（ACN）的生物活性。花色苷（ACN）的含量为 86.32±0.15%（w/w），被包裹于 AC N/CH/CS 纳米颗粒中，其粒径为 350.1±0.99nm（直径）和 42.55±0.54mV（Zeta 电位）。形态学研究和热重分析表明，ACN/CH/CS 纳米颗粒表现出异质形态和高的热稳定性。在 HCT-116 细胞系中，浓度为 0.05mg/ml 的 ACN 和 ACN/CH/CS 纳米颗粒分别使细胞凋亡增加了 12.1%和 35.1%，这表明纳米颗粒系统导致了细胞凋亡的显著增加（p<0.05）。ACN/CH/CS 纳米颗粒引起的线粒体结构变化表明，纳米颗粒对线粒体有负面影响。这些结果表明，纳米颗粒有可能作为一种载体系统来提高 ACN 的功效。
