Ashida Keita, Hotta Kohji, Oka Kotaro
Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University, Yokohama 223-8522, Japan.
Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University, Yokohama 223-8522, Japan; Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung City 80708, Taiwan; Waseda Research Institute for Science and Engineering, Waseda University, 2-2 Wakamatsucho, Shinjuku, Tokyo 162-8480, Japan.
iScience. 2019 Sep 27;19:191-203. doi: 10.1016/j.isci.2019.07.028. Epub 2019 Jul 23.
Determining how neurotransmitter input causes various neuronal activities is crucial to understanding neuronal information processing. In Caenorhabditis elegans, AIY interneurons receive several sources of sensory information as glutamate inputs and regulate behavior by integrating these inputs. However, the relationship between glutamate input and the Ca response in AIY under environmental noise, in other words, without explicit stimulation, remains unknown. Here, we show that glutamate-input fluctuations evoke a sporadic Ca response in AIY without stimulation. To ensure that Ca response can be considered AIY output, we show that the membrane-potential depolarization precedes Ca responses in AIY. We used an odor as model stimulation to modulate the sensory inputs. Simultaneous imaging of glutamate input and Ca response, together with glutamate transmission mutants, showed that glutamate-input fluctuations evoke sporadic Ca responses. We identified the input-output relationships under environmental noise in vivo, and our results address the relationship between sensory-input fluctuations and behavioral variability.
确定神经递质输入如何引发各种神经元活动对于理解神经元信息处理至关重要。在秀丽隐杆线虫中，AIY中间神经元接收多种作为谷氨酸输入的感觉信息来源，并通过整合这些输入来调节行为。然而，在环境噪声下，即在没有明确刺激的情况下，谷氨酸输入与AIY中的钙反应之间的关系仍然未知。在这里，我们表明谷氨酸输入波动在没有刺激的情况下会在AIY中引发零星的钙反应。为了确保钙反应可以被视为AIY的输出，我们表明膜电位去极化先于AIY中的钙反应。我们使用气味作为模型刺激来调节感觉输入。谷氨酸输入和钙反应的同步成像，以及谷氨酸传递突变体，表明谷氨酸输入波动会引发零星的钙反应。我们确定了体内环境噪声下的输入-输出关系，我们的结果揭示了感觉输入波动与行为变异性之间的关系。
