School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang, 453003, China; Henan Province Engineering Research Center of Horticultural Plant Resource Utilization and Germplasm Enhancement, Xinxiang, 453003, China.
Key Laboratory of Crop Water Use and Regulation, Ministry of Agriculture and rural affairs/Farmland Irrigation Research Institute, Chinese Academy of Agricultural Sciences, Xinxiang, 453002, China.
J Hazard Mater. 2019 Dec 15;380:120885. doi: 10.1016/j.jhazmat.2019.120885. Epub 2019 Jul 9.
Tetracycline is an antibiotic that frequently contaminates the environment. In this study, the growth and metabolites of ryegrass seedlings treated with tetracycline (0, 1, 10 or 100 mg/L) for 5 days were investigated. The results showed that the growth of ryegrass and the concentrations of carotenoid and chlorophyll decreased as the tetracycline concentration increased. Tetracycline increased the production of reactive oxygen species (ROS) and cell permeability and triggered mitochondrial membrane potential loss in the roots of ryegrass. The metabolic profiles of ryegrass differed between the control and tetracycline-treated groups. The contents of glucose, shikimic acid, aconitic acid, serine, lactose, phenylalanine, mannitol, galactose, gluconic acid, asparagine, and glucopyranose were positively correlated with root length and had high variable importance projection values. These compounds may have crucial functions in root extension. Tetracycline also affected aminoacyl-tRNA biosynthesis, nitrogen metabolism, and alanine, aspartate and glutamate metabolism in the roots. Tetracycline may affect root extension by regulating the synthesis/degradation of these metabolites or the activity of their biosynthetic pathways. These results provide an insight into the stress response of ryegrass to tetracycline.
四环素是一种常见的环境污染物。本研究采用水培法，以黑麦草幼苗为供试植物，研究了四环素（0、1、10 和 100 mg/L）对黑麦草幼苗生长及代谢物的影响。结果表明，随着四环素浓度的增加，黑麦草的生长、类胡萝卜素和叶绿素浓度降低。四环素增加了活性氧（ROS）的产生和细胞通透性，导致黑麦草根线粒体膜电位丧失。与对照组相比，四环素处理组黑麦草的代谢谱发生了变化。葡萄糖、莽草酸、乌头酸、丝氨酸、乳糖、苯丙氨酸、甘露醇、半乳糖、葡萄糖酸、天冬酰胺和吡喃葡萄糖的含量与根长呈正相关，且具有较高的变量重要性投影值。这些化合物可能在根伸长中起关键作用。四环素还影响了根中的氨基酸-tRNA 生物合成、氮代谢以及天冬氨酸、谷氨酸和丙氨酸代谢。四环素可能通过调节这些代谢物的合成/降解或其生物合成途径的活性来影响根的伸长。这些结果为黑麦草对四环素胁迫的响应机制提供了新的见解。
