Yang Yong, Jia Jiangtao, Han Tao, Zhang Heng, Wang Yvjie, Shao Luying, Wang Xinyi
Henan Joint International Laboratory for Crop Multi-Omics Research, School of Life Sciences, Henan University, No. 85 Jinming Road, Kaifeng 475004, China.
National Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Henan University, No. 85 Jinming Road, Kaifeng 475004, China.
Int J Mol Sci. 2025 Apr 30;26(9):4257. doi: 10.3390/ijms26094257.
Metabolomics is a commonly used method to study the responses of organisms to environmental changes. However, the relationships between metabolites and biological endpoints still need further discussion. In this study, we exposed wheat seeds to sulfamethoxazole (0, 1, 10, 100 mg/L) for 5 days. The results show that sulfamethoxazole (SMX) had an inhibitory effect on wheat growth. It reduced shoot length, root length, shoot fresh weight, root fresh weight, chlorophyll content, and carotenoid content. At the same time, it increased the concentration of reactive oxygen species, the activity of superoxide dismutase, the activity of peroxidase, and the activity of catalase in the root. An orthogonal partial least squares analysis and correlation analysis were performed. SMX transformed five key metabolic pathways. Notably, certain metabolic alterations exhibited negative correlations with reactive oxygen species (ROS) accumulation and antioxidant enzyme activities (including superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT)), while showing positive associations with root growth parameters (fresh weight and length). Conversely, other metabolic changes appeared to promote ROS generation and enhance antioxidant enzyme activities, consequently inhibiting root growth. These findings offer novel perspectives on the metabolic regulation of wheat's stress response to SMX exposure.
代谢组学是一种常用的研究生物体对环境变化反应的方法。然而，代谢物与生物学终点之间的关系仍需进一步探讨。在本研究中，我们将小麦种子暴露于磺胺甲恶唑（0、1、10、100毫克/升）中5天。结果表明，磺胺甲恶唑（SMX）对小麦生长有抑制作用。它降低了地上部长度、根长度、地上部鲜重、根鲜重、叶绿素含量和类胡萝卜素含量。同时，它增加了根部活性氧的浓度、超氧化物歧化酶的活性、过氧化物酶的活性和过氧化氢酶的活性。进行了正交偏最小二乘法分析和相关性分析。SMX改变了五个关键代谢途径。值得注意的是，某些代谢改变与活性氧（ROS）积累和抗氧化酶活性（包括超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT））呈负相关，而与根生长参数（鲜重和长度）呈正相关。相反，其他代谢变化似乎促进了ROS的产生并增强了抗氧化酶活性，从而抑制了根的生长。这些发现为小麦对SMX暴露应激反应的代谢调控提供了新的视角。
