Department of Rheumatology and Immunology, Arthritis Research Institute, the First Affiliated Hospital of Anhui Medical University, No. 218, Jixi Street, Hefei, Anhui, China.
Menzies Institute for Medical Research, University of Tasmania, 17 Liverpool Street, Hobart, Tasmania, Australia.
Clin Rheumatol. 2019 Dec;38(12):3609-3617. doi: 10.1007/s10067-019-04718-8. Epub 2019 Aug 3.
The aim of this study was to investigate cross-sectional associations between serum levels of IL-8 and the above outcomes in patients with knee osteoarthritis (OA).
A total of 160 subjects with clinical knee OA were included. Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) score and Lequesne index were used to assess the joint symptom. Magnetic resonance imaging was used to measure knee structural abnormalities including infrapatellar fat pad (IPFP) signal intensity alternation. Knee radiographic OA was assessed by radiography using the Kellgren-Lawrence (K-L) grading system. Enzyme-linked immunosorbent assay was used to measure the serum levels of IL-8 and cartilage or bone biomarkers.
In multivariable analyses, serum IL-8 was positively associated with WOMAC weight-bearing pain (β 2.85, P = 0.028), WOMAC physical dysfunction (β 12.71, P = 0.048), and Lequesne index (β 1.65, P = 0.015), and had positive associations with IPFP signal intensity alteration (OR 3.18, P = 0.011) and serum levels of N-telopeptide of type I collagen (NTXI), N-terminal procollagen III propeptide (PIIINP), matrix metalloproteinase (MMP)3, and MMP13 (β 0.24-1.44, all P < 0.05) in patients with clinical knee OA. Furthermore, there were positive associations between IL-8 and WOMAC score (β 22.49, P = 0.037), K-L grades (OR 3.88, P = 0.013), and IPFP signal intensity alteration (OR 3.20, P = 0.033) in patients with radiographic OA.
Serum levels of IL-8 were positively associated with increased knee symptoms, IPFP signal intensity alteration, and serum levels of bone and/or cartilage biomarkers, suggesting that IL-8 may have a role to play in knee OA.Key Point• This study systemically investigates the associations between serum IL 8 and knee symptoms, joint structures, and cartilage or bone biomarkers in patients with knee osteoarthritis, and some significant associations have been found, suggesting that IL 8 may have a role to play in knee OA.
本研究旨在探讨膝骨关节炎（OA）患者血清白细胞介素-8（IL-8）水平与上述结局的横断面相关性。
共纳入 160 例临床膝 OA 患者。采用西部安大略省和麦克马斯特大学骨关节炎指数（WOMAC）评分和 Lequesne 指数评估关节症状。磁共振成像（MRI）用于测量包括髌下脂肪垫（IPFP）信号强度改变在内的膝关节结构异常。膝关节放射学 OA 采用 Kellgren-Lawrence（K-L）分级系统进行放射学评估。酶联免疫吸附试验（ELISA）用于测量血清 IL-8 及软骨或骨生物标志物水平。
多变量分析显示，血清 IL-8 与 WOMAC 负重疼痛（β 2.85，P=0.028）、WOMAC 躯体功能障碍（β 12.71，P=0.048）和 Lequesne 指数（β 1.65，P=0.015）呈正相关，与 IPFP 信号强度改变（OR 3.18，P=0.011）及血清Ⅰ型胶原 N 端肽（NTXI）、Ⅲ型前胶原 N 端肽（PIIINP）、基质金属蛋白酶（MMP）3 和 MMP13 水平呈正相关（β 0.24-1.44，均 P<0.05）。此外，在影像学 OA 患者中，IL-8 与 WOMAC 评分（β 22.49，P=0.037）、K-L 分级（OR 3.88，P=0.013）和 IPFP 信号强度改变（OR 3.20，P=0.033）呈正相关。
血清 IL-8 水平与膝关节症状加重、IPFP 信号强度改变及血清骨和/或软骨生物标志物水平升高呈正相关，提示 IL-8 可能在膝 OA 中发挥作用。
本研究系统地研究了膝骨关节炎患者血清白细胞介素-8（IL-8）与膝关节症状、关节结构及软骨或骨生物标志物之间的相关性，发现了一些有意义的相关性，提示 IL-8 可能在膝骨关节炎中发挥作用。
