Department of Oral and Maxillofacial Surgery, College of Dentistry, Yonsei University, 50-1, Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.
Department of Oral and Maxillofacial Surgery, Wonkwang University Sanbon Hospital, 321, Sanbon-ro, Gunpo-si, Gyeonggi-do, 15865, Republic of Korea.
Neurochem Res. 2019 Sep;44(9):2092-2102. doi: 10.1007/s11064-019-02846-w. Epub 2019 Aug 3.
The aim of this study was to evaluate the diagnostic efficacy of F-FDG PET/MRI in two different peripheral neuropathic pain models using the injured rat sciatic nerves. Twelve rats, with operation on left sciatic nerves, were evenly divided into three groups: sham surgery (control group), crushing injury and chronic constriction injury (CCI) (experimental groups). The nerve damage was assessed at 3 weeks postoperatively using following methods: paw withdrawal threshold values (RevWT), maximum standardized uptake values on PET/MRI images (SUVR), and counting the number of myelinated axons in proximal and distal sites of nerve injury (MAxR). The results were quantified and statistically analyzed. Compared to the control group, the crushing injury demonstrated significant differences in RevWT (p < 0.0001) and SUVR (p = 0.027) and the CCI group demonstrated significant differences in RevWT (p < 0.0001), SUVR (p = 0.001) and MAxR (p = 0.048). There were no significant differences between the two experimental groups for all assessments. Correlation analysis demonstrated that RevWT and SUVR assessments were highly correlated (r = -- 0.710, p = 0.010), and SUVR and MAxR were highly correlated (r = 0.611, p = 0.035). However, there was no significant correlation between RevWT and MAxR. The PET scan may be a valuable imaging modality to enable noninvasive, objective diagnosis of neuropathic pain caused by peripheral nerve injury. Also, MRI fused with PET may help clarify the anatomic location of soft tissue structures, including the peripheral nerves.
本研究旨在使用损伤大鼠坐骨神经评估 F-FDG PET/MRI 在两种不同周围神经性疼痛模型中的诊断效能。12 只大鼠的左侧坐骨神经接受手术，平均分为三组：假手术（对照组）、挤压损伤和慢性缩窄性损伤（CCI）（实验组）。术后 3 周，采用以下方法评估神经损伤：足底缩足反射阈值（RevWT）、PET/MRI 图像最大标准化摄取值（SUV）和神经损伤近端和远端有髓轴突计数（MAxR）。对结果进行量化和统计学分析。与对照组相比，挤压损伤组 RevWT（p<0.0001）和 SUV（p=0.027）有显著差异，CCI 组 RevWT（p<0.0001）、SUV（p=0.001）和 MAxR（p=0.048）有显著差异。所有评估中两组实验之间均无显著差异。相关性分析表明，RevWT 和 SUV 评估高度相关（r=-0.710，p=0.010），SUV 和 MAxR 高度相关（r=0.611，p=0.035）。然而，RevWT 和 MAxR 之间无显著相关性。PET 扫描可能是一种有价值的成像方式，可实现对周围神经损伤引起的神经性疼痛的非侵入性、客观诊断。此外，与 PET 融合的 MRI 可能有助于阐明软组织结构（包括周围神经）的解剖位置。
