Lee Hye Yeong, Lee Hye-Lan, Yun Yeomin, Kim Jin-Su, Ha Yoon, Yoon Do Heum, Lee Soo-Hong, Shin Dong Ah
1 Department of Neurosurgery, Spine & Spinal Cord Institute, Yonsei University , Seoul, Republic of Korea.
2 Brain Korea 21 PLUS Project for Medical Science, College of Medicine, Yonsei University , Seoul, Republic of Korea.
Tissue Eng Part A. 2015 Jul;21(13-14):2044-52. doi: 10.1089/ten.TEA.2014.0713. Epub 2015 May 20.
Stem cells are a promising source of tissue engineering due to their differentiation potential. Today, direct transplantation of stem cells for cell therapy is commonly performed. However, in cases of nerve injury, direct transplantation of cells could lead to secondary nerve damage. Male Sprague-Dawley rats were randomized into four groups: the phosphate-buffered saline epineural transplantation (PBS-ENT) group, the PBS intraneural transplantation (PBS-INT) group, the human adipose-derived stem cells epineural transplantation (hASCs-ENT) group, and human adipose-derived stem cells intraneural transplantation (hASCs-INT) group. Transplantation was conducted 1 week later after inflicting a crush injury with subsequent observation for 5 weeks. To evaluate pain, each group was examined with regard to paw withdrawal latency and evoked potentials. The sciatic functional index (SFI) was calculated to estimate functional recovery. The sciatic nerve was also examined histologically. The hASCs-ENT group showed a more rapid paw withdrawal threshold and SFI recovery than the other groups (p<0.05). The hASCs-ENT group also showed shorter initial latencies in both somatosensory evoked potential (SSEP) and motor evoked potential (MEP) than the PBS-INT group (p<0.05). In addition, the N1 latency of the MEP and the N1 and P1 latencies of the SSEP were significantly shorter than those of the PBS-INT group (p<0.05). Histological examination revealed that the transplanted groups showed better neural recovery and remyelination than the groups injected with PBS. These results show that the transplantation of hASCs into the injured sciatic nerve improved mechanical allodynia and functional recovery as determined by the paw withdrawal test, SFI analysis, and electrophysiological studies. ENT is superior to INT in terms of invasiveness and better outcomes.
由于干细胞具有分化潜能，它们是组织工程中一种很有前景的来源。如今，干细胞直接移植用于细胞治疗已普遍开展。然而，在神经损伤的情况下，细胞直接移植可能会导致继发性神经损伤。将雄性Sprague-Dawley大鼠随机分为四组：磷酸盐缓冲盐水外膜移植（PBS-ENT）组、PBS神经内移植（PBS-INT）组、人脂肪源性干细胞外膜移植（hASCs-ENT）组和人脂肪源性干细胞神经内移植（hASCs-INT）组。在造成挤压伤1周后进行移植，随后观察5周。为了评估疼痛情况，对每组大鼠进行爪部撤离潜伏期和诱发电位检查。计算坐骨神经功能指数（SFI）以评估功能恢复情况。还对坐骨神经进行了组织学检查。hASCs-ENT组的爪部撤离阈值和SFI恢复比其他组更快（p<0.05）。hASCs-ENT组的体感诱发电位（SSEP）和运动诱发电位（MEP）的初始潜伏期也比PBS-INT组短（p<0.05）。此外，MEP的N1潜伏期以及SSEP的N1和P1潜伏期均显著短于PBS-INT组（p<0.05）。组织学检查显示，移植组的神经恢复和髓鞘再生情况优于注射PBS的组。这些结果表明，将hASCs移植到损伤的坐骨神经中可改善机械性异常性疼痛和功能恢复，这通过爪部撤离试验、SFI分析和电生理研究得以确定。在外伤性和更好的结果方面，外膜移植优于神经内移植。
