Keenan J I, Aitchison A, Pearson J F, Frizelle F A, Munday J S
Department of Surgery, University of Otago Christchurch , Christchurch , New Zealand.
Biostatistics and Computational Biology Unit, University of Otago Christchurch , Christchurch , New Zealand.
N Z Vet J. 2019 Nov;67(6):329-332. doi: 10.1080/00480169.2019.1651233. Epub 2019 Sep 4.
To determine if presence of the toxin () gene, a molecular marker of colonic carriage of entertoxigenic (ETBF) in humans, was associated with a finding of small intestinal adenocarcinomas (SIA) in sheep in New Zealand. Samples of jejunal tissue were collected from the site of tumours and from grossly normal adjacent tissue in 20 sheep, in different consignments, diagnosed with SIA based on gross examination of viscera following slaughter. Two jejunal samples were also collected from a control sheep in the same consignment that had no gross evidence of SIA. A PCR assay was used to detect the presence of the gene in the samples. Of the sheep with SIA, the gene was amplified from one or both samples from 7/20 (35%) sheep, and in sheep that had no gross evidence of SIA the gene was amplified from at least one sample in 11/20 (55%) sheep (RR 0.61; 95% CI = 0.30-1.25; p = 0.34). Of 11 positive samples analysed, ETBF subtype -1 was detected in one, -2 was detected in 10, and none were -3. There was a high prevalence of detection of the gene in both SIA-affected and non-affected sheep, but there was no apparent association between carriage of ETBF, evidenced by detection of the gene, and the presence of SIA. ETBF are increasingly implicated in the aetiology of human colorectal cancer, raising the possibility that sheep may provide a zoonotic reservoir of this potentially carcinogenic bacterium. : toxin; ETBF: Enterotoxigenic ; SIA: Small intestinal adenocarcinoma.
为了确定毒素（）基因（人类产肠毒素性脆弱拟杆菌（ETBF）结肠携带的分子标志物）的存在是否与新西兰绵羊小肠腺癌（SIA）的发现相关。从20只绵羊的肿瘤部位和大体正常的相邻组织中采集空肠组织样本，这些绵羊来自不同批次，在屠宰后通过内脏大体检查诊断为SIA。还从同一批次的一只无SIA大体证据的对照绵羊中采集了两个空肠样本。采用聚合酶链反应（PCR）检测法检测样本中基因的存在情况。在患有SIA的绵羊中，7/20（35%）的绵羊一个或两个样本中扩增出了基因，而在无SIA大体证据的绵羊中，11/20（55%）的绵羊至少一个样本中扩增出了基因（相对风险0.61；95%置信区间=0.30 - 1.25；p = 0.34）。在分析的11个阳性样本中，检测到ETBF亚型-1的有1个，-2的有10个，无-3的。在受SIA影响和未受影响的绵羊中基因检测的患病率都很高，但通过基因检测证明的ETBF携带与SIA的存在之间没有明显关联。ETBF越来越多地被认为与人类结直肠癌的病因有关，这增加了绵羊可能是这种潜在致癌细菌人畜共患病储存宿主的可能性。：毒素；ETBF：产肠毒素性；SIA：小肠腺癌。
