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在脆弱拟杆菌肠道外分离株中鉴定出第三个金属蛋白酶毒素基因。

Identification of a third metalloprotease toxin gene in extraintestinal isolates of Bacteroides fragilis.

作者信息

Chung G T, Franco A A, Wu S, Rhie G E, Cheng R, Oh H B, Sears C L

机构信息

Laboratory of Bacterial Toxins, Department of Microbiology, National Institute of Health, Seoul, Korea.

出版信息

Infect Immun. 1999 Sep;67(9):4945-9. doi: 10.1128/IAI.67.9.4945-4949.1999.

DOI:10.1128/IAI.67.9.4945-4949.1999
PMID:10456956
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC96834/
Abstract

To further understand the epidemiology of enterotoxigenic Bacteroides fragilis (ETBF), 89 extraintestinal B. fragilis strains from Seoul, Korea, were examined for secretion of B. fragilis toxin (BFT) by the HT29/C1 biologic assay and for the B. fragilis toxin gene (bft) by colony blot hybridization and PCR. Complete agreement between the three techniques was found. Overall, 34 B. fragilis strains (38%) were identified as ETBF. Eleven of the 34 ETBF strains (32%) expressed a new isoform of BFT (Korea-BFT). This new isoform is more related to BFT-2 than to BFT-1. Like BFT-1 and BFT-2, Korea-BFT cleaves E-cadherin, the zonula adherens protein.

摘要

为进一步了解产肠毒素脆弱拟杆菌（ETBF）的流行病学情况，采用HT29/C1生物学检测法对来自韩国首尔的89株肠道外脆弱拟杆菌菌株进行脆弱拟杆菌毒素（BFT）分泌检测，并通过菌落杂交和聚合酶链反应（PCR）检测脆弱拟杆菌毒素基因（bft）。结果发现这三种技术完全一致。总体而言，34株脆弱拟杆菌菌株（38%）被鉴定为ETBF。34株ETBF菌株中有11株（32%）表达了一种新的BFT同工型（韩国-BFT）。这种新的同工型与BFT-2的关系比与BFT-1的关系更密切。与BFT-1和BFT-2一样，韩国-BFT可切割E-钙黏蛋白，即黏着小带蛋白。

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本文引用的文献

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Bacteroides fragilis enterotoxin cleaves the zonula adherens protein, E-cadherin.脆弱拟杆菌肠毒素可切割黏着小带蛋白E-钙黏蛋白。
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Bacteroides fragilis toxin exhibits polar activity on monolayers of human intestinal epithelial cells (T84 cells) in vitro.脆弱拟杆菌毒素在体外对人肠上皮细胞（T84细胞）单层具有极性活性。
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Bacteroides fragilis toxin rearranges the actin cytoskeleton of HT29/C1 cells without direct proteolysis of actin or decrease in F-actin content.脆弱拟杆菌毒素可重排HT29/C1细胞的肌动蛋白细胞骨架，而不会直接对肌动蛋白进行蛋白水解或降低F-肌动蛋白含量。
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Cloning and characterization of the Bacteroides fragilis metalloprotease toxin gene.脆弱拟杆菌金属蛋白酶毒素基因的克隆与鉴定
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Cloning and characterization of the gene for the metalloprotease enterotoxin of Bacteroides fragilis.脆弱拟杆菌金属蛋白酶肠毒素基因的克隆与鉴定
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