Autran-Martínez M, Cortes-Acevedo P, Cerón-Gutiérrez L, Davies S, Mendoza-Elvira E S, Martínez-Rodríguez H A, Liboni C, Pérez-Valencia V, Döffinger R, Barcenas-Morales G
Laboratorio 2 Inmunología, Facultad de Estudios Superiores (FES) Cuautitlán, Universidad Nacional Autonoma de México (UNAM), Mexico.
Department of Clinical Biochemistry and Immunology, Addenbrookes Hospital and National Institute of Health Research (NIHR), Cambridge Biomedical Research Centre, Cambridge, UK.
Vet Immunol Immunopathol. 2019 Aug;214:109902. doi: 10.1016/j.vetimm.2019.109902. Epub 2019 Jul 15.
Autoantibodies against cytokines have been associated with immunodeficiency, susceptibility to infectious diseases, autoimmunity and inflammation in humans, but have not yet been investigated in the Veterinary field so far. The aim of the current study was to determine the presence of anti-cytokine autoantibodies in canines suffering from various conditions including recurrent infections, autoimmune diseases and cancer in comparison to healthy controls. This is the first report of the presence of autoantibodies against cytokines in dogs. A total of 101 serum samples (51 patients and 50 clinically healthy dogs) from the state of Mexico and surroundings were analysed using a multiplex bead-based flow cytometry assay. Results show significant levels of various anti-cytokine autoantibodies in diseased dogs but not in healthy controls. In addition we show distinct associations of various disease types to the specificity of anti-cytokine autoantibodies and to response complexities. Apart from the direct functional/causal implication of anti-cytokine auto-antibodies on disease processes, this findings point to the possibility to use anti-cytokine response patterns as diagnostic tools.
针对细胞因子的自身抗体与人类的免疫缺陷、传染病易感性、自身免疫和炎症有关，但迄今为止尚未在兽医领域进行研究。本研究的目的是确定与健康对照相比，患有各种疾病（包括反复感染、自身免疫性疾病和癌症）的犬类中抗细胞因子自身抗体的存在情况。这是关于犬类中存在抗细胞因子自身抗体的首次报告。使用基于多重微珠的流式细胞术分析方法，对来自墨西哥州及周边地区的101份血清样本（51例患者和50只临床健康的犬）进行了分析。结果显示，患病犬体内存在显著水平的各种抗细胞因子自身抗体，而健康对照中则没有。此外，我们还展示了各种疾病类型与抗细胞因子自身抗体特异性及反应复杂性之间的明显关联。除了抗细胞因子自身抗体对疾病过程的直接功能/因果影响外，这些发现还指出了将抗细胞因子反应模式用作诊断工具的可能性。
