Kurth C D, Wagerle L C, Delivoria-Papadopoulos M
Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia 19104.
Am J Physiol. 1988 Sep;255(3 Pt 2):H563-8. doi: 10.1152/ajpheart.1988.255.3.H563.
We examined cerebral blood flow (CBF) regulation by the sympathetic nerves in 12 newborn lambs (3-11 days old) during seizures, a potent reflex stimulator of the sympathetic nervous system. CBF was measured with microspheres, and seizures were induced with bicuculline. In six of these lambs, one hemibrain was denervated (D) chronically by interrupting the ipsilateral cervical sympathetic trunk; the other hemibrain remained innervated (I). Before and after 10, 35, and 70 min of seizures, cerebral gray matter blood flow (mean +/- SE ml.min-1.100 g-1) was, respectively, 12 +/- 3 (9%), 71 +/- 12 (21%), 120 +/- 15 (38%), and 54 +/- 5 (14%) greater (P less than 0.05) in the D than in the I hemibrain. In the cerebral white matter, hippocampus, caudate, and thalamus blood flows to the D and I hemibrains were similar before seizures but during seizures they were 10-39% greater (P less than 0.05) in the D than in the I hemibrain. Midbrain, brainstem, and cerebellum D and I blood flows were always similar. In the other six lambs, acute denervation during seizures increased ipsilateral cerebral gray and hippocampus blood flow by 10-31%, but unilateral electrical stimulation decreased ipsilateral cerebral gray, cerebral white, hippocampus, thalamus, and caudate blood flow by 17-27%. The data demonstrate that, during seizures, sympathetic nerve activity modifies regional CBF and the effect is sustained, suggesting a role for the sympathetic nervous system in newborn CBF regulation.
我们在12只新生羔羊（3 - 11日龄）癫痫发作期间研究了交感神经对脑血流量（CBF）的调节，癫痫发作是交感神经系统的一种强烈反射刺激。用微球测量CBF，并用荷包牡丹碱诱发癫痫发作。在其中6只羔羊中，通过切断同侧颈交感干对一侧半脑进行慢性去神经支配（D）；另一侧半脑保持神经支配（I）。在癫痫发作10、35和70分钟之前和之后，脑灰质血流量（平均值±标准误，ml·min⁻¹·100g⁻¹）在去神经支配的半脑（D）分别比有神经支配的半脑（I）高12±3（9%）、71±12（21%）、120±15（3 / 8%）和54±5（14%）（P＜0.05）。在脑白质、海马、尾状核和丘脑中，癫痫发作前去神经支配和有神经支配的半脑血流量相似，但在癫痫发作期间，去神经支配的半脑比有神经支配的半脑高10 - 39%（P＜0.05）。中脑、脑干和小脑的去神经支配和有神经支配的半脑血流量始终相似。在另外6只羔羊中，癫痫发作期间急性去神经支配使同侧脑灰质和海马血流量增加10 - 31%，但单侧电刺激使同侧脑灰质、脑白质、海马、丘脑和尾状核血流量减少17 - 27%。数据表明，在癫痫发作期间，交感神经活动改变局部CBF，且这种作用持续存在，提示交感神经系统在新生动物CBF调节中起作用。
