Department of Food Science, University of Guelph, Guelph N1G2W1, ON, Canada.
Department of Animal Biosciences, University of Guelph, Guelph N1G2W1, ON, Canada.
J Dairy Sci. 2019 Oct;102(10):8604-8613. doi: 10.3168/jds.2018-15786. Epub 2019 Aug 1.
The objective of this work was to better understand the effect of differences in milk protein composition, and specifically, a change in β-casein to total casein in a milk-based matrix, on growth performance and metabolic and inflammatory responses using a piglet model. Three formulas were optimized for piglets, with similar metabolizable energy, total protein content, and other essential nutrients. Only the protein type and ratio varied between the treatments: the protein fraction of the control diet contained only whey proteins, whereas 2 other matrices contained a whey protein to casein ratio of 60:40, and differed in the amount of β-casein (12.5 and 17.1% of total protein). Piglets fed formula containing whey proteins and caseins, regardless of the concentration of β-casein, showed a significantly higher average daily gain, average daily feed intake, and feed efficiency compared with piglets consuming the formula with only whey protein. Consumption of the formula containing only whey protein showed higher levels of plasma glucagon-like peptide-1 and ghrelin compared with the consumption of formula containing casein and whey protein. A positive correlation was observed between postprandial time and glucagon-like peptide-1 response. The intestinal pro-inflammatory cytokine tumor necrosis factor α increased significantly in piglets fed the whey protein/casein diet compared with those fed whey protein formula. All formula-fed piglets showed a lower level of IL-6 cytokine compared with the ad libitum sow-fed piglets, regardless of composition. No significant differences in the anti-inflammatory IL-10 concentration were observed between treatment groups. Milk protein composition contributed to the regulation of piglets' metabolic and physiological responses, with whey protein/casein formula promoting growth performance and a different immune regulatory balance compared with a formula containing only whey protein. Results indicated no differences between treatments containing different levels of β-casein.
本研究旨在通过仔猪模型，更好地理解乳蛋白组成差异（特别是乳基基质中β-酪蛋白与总酪蛋白比例的变化）对生长性能及代谢和炎症反应的影响。三种配方均针对仔猪进行了优化，其代谢能、总蛋白含量和其他必需营养素均相似。仅处理间的蛋白质类型和比例存在差异：对照饮食的蛋白质部分仅含有乳清蛋白，而其他两种基质的乳清蛋白与酪蛋白的比例为 60:40，且β-酪蛋白的含量不同（分别占总蛋白的 12.5%和 17.1%）。与仅摄入乳清蛋白的仔猪相比，摄入含有乳清蛋白和酪蛋白的配方的仔猪，其平均日增重、平均日采食量和饲料效率均显著提高，无论β-酪蛋白的浓度如何。与摄入含有乳清蛋白和酪蛋白的配方的仔猪相比，摄入仅含有乳清蛋白的配方的仔猪的血浆胰高血糖素样肽-1和胃饥饿素水平更高。餐后时间与胰高血糖素样肽-1反应呈正相关。与摄入乳清蛋白配方的仔猪相比，摄入乳清蛋白/酪蛋白配方的仔猪的肠道促炎细胞因子肿瘤坏死因子-α显著增加。与自由采食母猪喂养的仔猪相比，所有配方喂养的仔猪的白细胞介素-6 细胞因子水平均较低，无论其组成如何。各组间抗炎白细胞介素-10 浓度无显著差异。乳蛋白组成影响仔猪的代谢和生理反应，乳清蛋白/酪蛋白配方与仅含有乳清蛋白的配方相比，可促进生长性能和不同的免疫调节平衡。结果表明，不同β-酪蛋白水平的处理间无差异。
