a Department of VIP Center and Shandong Provincial Key Laboratory of Oral Biomedicine, School and Hospital of Stomatology, Shandong University , Jinan , Shandong , China.
b Department of Endodontics, Jinan Stomatological Hospital , Jinan , Shandong , China.
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):3278-3285. doi: 10.1080/21691401.2019.1647222.
The current study aimed to explore the function of on the progression of oral squamous cell carcinoma (OSCC) and relevant molecular mechanisms. Expression profile of in OSCC tissues and cells was detected using quantitative real-time polymerase chain reaction (qRT-PCR) method. Chi-square test was performed to estimate the relationship between and clinical parameters of OSCC cases. Cell viability and motility abilities were estimated using MTT and transwell assays, respectively. Potential targets of in OSCC were identified through bioinformatics analysis and luciferase reporter assay. exhibited decreased expression in OSCC tissues and cells, compared to non-cancerous controls ( < .05 for both). Moreover, its down-regulation was closely correlated with lymph node metastasis ( = .044) and TNM stages ( = .001). Enforced expression reduced cell proliferation, migration and invasion, while its knockdown exhibited opposite effects. Phospholipase D1 () was confirmed as a target of in OSCC. could inhibit wnt/β-catenin pathway through targeting , thus realizing its anti-tumour action in OSCC. may be a tumour suppressor in OSCC by targeting /Wnt/β-catenin pathway.
本研究旨在探讨 在口腔鳞状细胞癌（OSCC）进展中的作用及其相关分子机制。采用实时定量聚合酶链反应（qRT-PCR）方法检测 在 OSCC 组织和细胞中的表达谱。采用卡方检验估计 与 OSCC 病例临床参数之间的关系。通过 MTT 和 Transwell 测定分别评估细胞活力和迁移能力。通过生物信息学分析和荧光素酶报告基因实验鉴定 在 OSCC 中的潜在靶标。与非癌对照相比， 在 OSCC 组织和细胞中表达下调（均<0.05）。此外，其下调与淋巴结转移（=0.044）和 TNM 分期（=0.001）密切相关。过表达 可降低细胞增殖、迁移和侵袭能力，而敲低则表现出相反的效果。磷脂酶 D1 () 被确认为 OSCC 中 的靶标。通过靶向 /Wnt/β-catenin 通路， 可抑制 wnt/β-catenin 通路，从而在 OSCC 中发挥其抗肿瘤作用。 可能通过靶向 /Wnt/β-catenin 通路在 OSCC 中作为肿瘤抑制因子。
