a Department of Respiration, Daping Hospital, Army Military Medical University , Chongqing , China.
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):3272-3277. doi: 10.1080/21691401.2019.1647221.
To evaluate the diagnostic effect of sequential detection of Adenosine deaminase (ADA) screening and T-SPOT assay on tuberculosis (TB) pleurisy in pleural effusion patients. 248 pleural effusion patients (172 TB and 76 non-TB) were retrospectively analyzed in the study. The concentrations of ADA and lactate dehydrogenase (LDH) were measured in pleural fluids and serum samples of the patients. T-SPOTT assays were performed in pleural fluids. The relationship between ADA, T-SPOT and the occurrence of TB pleurisy was evaluated using logistic regression analysis. The level of pleural ADA and positive rate of T-SPOT were all higher in TB pleurisy group than non-TB pleurisy group ( < .001). The positive rate of T-SPOT detection reached 98.83% in the TB pleurisy group while only 40.7% in non-TB pleurisy group ( < .001). Additionally, 8 patients (4.65%) in the TB pleurisy group showed the level of pleurisy ADA exceeded 40 IU/L while only one patient (1.31%) in the non-TB pleurisy group. The sequential detection of ADA screening and T-SPOT assay was found to be an accurate and rapid method for identifying TB pleurisy from pleural effusion, which would promote effective treatment.
为了评估腺苷脱氨酶（ADA）筛查和 T-SPOT 检测在胸腔积液患者结核性胸膜炎中的诊断效果。本研究回顾性分析了 248 例胸腔积液患者（172 例结核性胸膜炎和 76 例非结核性胸膜炎）。测量患者胸腔积液和血清样本中的 ADA 和乳酸脱氢酶（LDH）浓度。在胸腔积液中进行 T-SPOTT 检测。使用逻辑回归分析评估 ADA、T-SPOT 与结核性胸膜炎发生的关系。结核性胸膜炎组胸腔 ADA 水平和 T-SPOT 阳性率均高于非结核性胸膜炎组（ < .001）。结核性胸膜炎组 T-SPOT 检测的阳性率达到 98.83%，而非结核性胸膜炎组仅为 40.7%（ < .001）。此外，结核性胸膜炎组有 8 例（4.65%）患者胸腔 ADA 水平超过 40IU/L，而非结核性胸膜炎组仅有 1 例（1.31%）患者。ADA 筛查和 T-SPOT 检测的序贯检测是一种准确、快速的方法，可用于从胸腔积液中识别结核性胸膜炎，从而促进有效治疗。
