少动假单胞菌Q1的多氯联苯降解菌株中2,3-二羟基联苯双加氧酶基因的克隆及核苷酸序列

Cloning and nucleotide sequence of the 2,3-dihydroxybiphenyl dioxygenase gene from the PCB-degrading strain of Pseudomonas paucimobilis Q1.

作者信息

Taira K, Hayase N, Arimura N, Yamashita S, Miyazaki T, Furukawa K

机构信息

Fermentation Research Institute, MITI, Tsukuba Science City, Ibaraki, Japan.

出版信息

Biochemistry. 1988 May 31;27(11):3990-6. doi: 10.1021/bi00411a015.

DOI:10.1021/bi00411a015

PMID:3137968

Abstract

The bphC gene encoding 2,3-dihydroxybiphenyl dioxygenase was cloned from biphenyl-degrading and chlorinated biphenyl-degrading Pseudomonas paucimobilis Q1, and its complete nucleotide sequence was determined. The DNA-derived protein sequence provides the primary structure of 298 amino acids. Polyclonal antibodies raised against this protein from P. paucimobilis Q1 failed to cross-react with the previously isolated 2,3-dihydroxybiphenyl dioxygenase from Pseudomonas pseudoalcaligenes KF707 [Furukawa, K., & Arimura, N. (1987) J. Bacteriol. 169, 924-927. Furukawa, K., Arimura, N., & Miyazaki, T. (1987) J. Bacteriol. 169, 427-429], despite the close similarities of these proteins in terms of their native as well as subunit molecular weights, cofactor, and enzymatic activities. The sequence homology of the 2,3-dihydroxybiphenyl dioxygenase from the two different sources is examined.

摘要

从能降解联苯和氯化联苯的少动假单胞菌Q1中克隆出了编码2,3-二羟基联苯双加氧酶的bphC基因，并测定了其完整的核苷酸序列。由DNA推导的蛋白质序列显示该蛋白由298个氨基酸组成。用少动假单胞菌Q1的这种蛋白制备的多克隆抗体，无法与之前从产碱假单胞菌KF707中分离出的2,3-二羟基联苯双加氧酶发生交叉反应[古川，K.，&有村，N.（1987年）《细菌学杂志》169, 924 - 927页。古川，K.，有村，N.，&宫崎，T.（1987年）《细菌学杂志》169, 427 - 429页]，尽管这两种蛋白质在天然状态以及亚基分子量、辅因子和酶活性方面都非常相似。对来自两种不同来源的2,3-二羟基联苯双加氧酶的序列同源性进行了检测。