Heidari Nasirabadi Mahboobeh, Shirazi Abolfazl, Kadivar Ali, Shams-Esfandabadi Naser, Mohebbi Abdolnaser, Ahmadi Ebrahim
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran.
Avicenna J Med Biotechnol. 2019 Jul-Sep;11(3):245-252.
In the process of sperm cryopreservation, apart from cryoinjury, the production of Reactive Oxygen Species (ROS) can adversely affect the integrity of chromatin and cellular membranes. Addition of natural antioxidants to freezing medium is an approach to reduce the destructive effects of ROS on sperm.
In this study, during 60 of cooling process, the ejaculates of five stallions were diluted in the following media: INRA 82 medium as Control (C), INRA 82 medium supplemented with 0.25% Sericin (S), INRA 82 medium supplemented with 1.5 Glutathione (G), and INRA 82 medium supplemented with 0.25% Sericin+1.5 Glutathione (S+G).
In the frozen/thawed sericin supplemented group, while the integrity of DNA and the activity of catalase and Glutathione Peroxidase (GPx) were increased, the lipid peroxidation and midpieceab normality decreased, compared with other groups (p<0.05). The proportions of sperms with abnormal head in group S and the sperm with distal droplet in G and S+G groups decreased, compared with group C (p<0.05). In CTC assay, the percentage of capacitated spermatozoa in treatment groups was lower than control (p<0.01).
In conclusion, the presence of sericin in freezing medium of stallion semen could improve sperm DNA integrity and its resistance to ROS and lipid peroxidation.
在精子冷冻保存过程中，除了冷冻损伤外，活性氧（ROS）的产生会对染色质和细胞膜的完整性产生不利影响。在冷冻培养基中添加天然抗氧化剂是减少ROS对精子破坏作用的一种方法。
在本研究中，在60个冷却过程中，将五匹种马的精液稀释于以下培养基中：作为对照的INRA 82培养基（C）、添加0.25%丝胶蛋白的INRA 82培养基（S）、添加1.5谷胱甘肽的INRA 82培养基（G）以及添加0.25%丝胶蛋白 + 1.5谷胱甘肽的INRA 82培养基（S + G）。
与其他组相比，在冷冻/解冻的添加丝胶蛋白组中，DNA完整性、过氧化氢酶和谷胱甘肽过氧化物酶（GPx）活性增加，脂质过氧化和中段异常减少（p < 0.05）。与C组相比，S组头部异常精子的比例以及G组和S + G组具有远侧小滴精子的比例降低（p < 0.05）。在CTC分析中，处理组中获能精子的百分比低于对照组（p < 0.01）。
总之，种马精液冷冻培养基中丝胶蛋白的存在可改善精子DNA完整性及其对ROS和脂质过氧化的抗性。
