Bromfield Eden S P, Cloutier Sylvie, Robidas Catherine, Tran Thi Thu Van, Darbyshire Stephen J
Ottawa Research and Development Centre Agriculture and Agri-Food Canada Ottawa Ontario Canada.
Ecol Evol. 2019 May 26;9(12):6999-7004. doi: 10.1002/ece3.5266. eCollection 2019 Jun.
The toxic legume plant, is native to the Eastern Mediterranean and Black Sea regions. This legume is considered to be a noxious weed, and its establishment in Canada may have resulted from ornamental planting and/or field trials. In its native range, a highly specific nitrogen-fixing symbiosis with the bacterium, symbiovar (sv.) officinalis, is required for normal growth. In North America, nothing is known about the bacterial symbionts of . Our purpose was to determine the species and symbiovar identity of symbiotic bacteria associated with invasive plants of at five sites in the province of Ontario, Canada. Sequence analysis of four housekeeping (16S rRNA, D, II, and A) and two symbiosis (C and H) genes showed that all 50 bacterial isolates from root nodules of at the five Canadian sites were identical to strains of sv. officinalis originating either from Europe or the Caucasus. Plant tests indicated that soils collected from four Canadian sites without a history of agriculture or presence of were deficient in symbiotic bacteria capable of eliciting nodules on this plant. Collectively our data support the hypothesis of anthropogenic co-introduction of and its specific symbiotic bacterium into Canada from the Old World. Factors that may limit the spread of in new environments are discussed.
这种有毒豆科植物原产于东地中海和黑海地区。这种豆科植物被视为有害杂草，它在加拿大的出现可能源于观赏种植和/或田间试验。在其原生范围内，它与药用共生菌共生变种（sv.）officinalis形成高度特异性的固氮共生关系，才能正常生长。在北美，对于该植物的细菌共生体一无所知。我们的目的是确定加拿大安大略省五个地点与该入侵植物相关的共生细菌的种类和共生变种身份。对四个持家基因（16S rRNA、D、II和A）和两个共生基因（C和H）的序列分析表明，从加拿大五个地点的该植物根瘤中分离出的所有50株细菌均与源自欧洲或高加索地区的药用共生菌共生变种菌株相同。植物试验表明，从加拿大四个没有农业历史或不存在该植物的地点采集的土壤中，缺乏能够在这种植物上引发根瘤的共生细菌。我们的数据共同支持了该植物及其特定共生细菌从旧世界人为共同引入加拿大的假说。文中还讨论了可能限制该植物在新环境中传播的因素。
