Radeva G, Jurgens G, Niemi M, Nick G, Suominen L, Lindström K
Department of Applied Chemistry and Microbiology, University of Helsinki, Finland.
Syst Appl Microbiol. 2001 Jul;24(2):192-205. doi: 10.1078/0723-2020-00029.
Twenty-six Rhizobium galegae strains, representing the center of origin of the host plants Galega orientalis and G. officinalis as well as other geographic regions, were used in a polyphasic analysis of the relationships of R. galegae strains. Phage typing, lipopolysaccharide (LPS) profiling, pulsed field gel electrophoresis (PFGE) profiling and rep-PCR (use of repetitive sequences as PCR primers for genomic fingerprinting) with REP and ERIC primers investigated nonsymbiotic properties, whereas plasmid profiling and hybridisation with a nif gene probe, and with nodB, nodD, nod box and an IS sequence from the symbiotic region as probes, were used to reveal the relationships of symbiotic genes. The results were used in pairwise calculations of distances between the strains, and the distances were visualised as a dendrogram. Indexes of association were compared for all tests pooled, and for chromosomal tests and symbiotic markers separately, to display the input of the different categories of tests on the grouping of the strains. Our study shows that symbiosis related genetic traits in R. galegae divide strains belonging to the species into two groups, which correspond to strains forming an effective symbioses with G. orientalis and G. officinalis respectively. We therefore propose that Rhizobium galegae strains forming an effective symbiosis with Galega orientalis are called R. galegae bv. orientalis and strains forming an effective symbiosis with Galega officinalis are called R. galegae bv. officinalis.
选用了26株代表宿主植物东方山羊豆和药用山羊豆的起源中心以及其他地理区域的加氏根瘤菌菌株，对加氏根瘤菌菌株间的关系进行多相分析。用噬菌体分型、脂多糖（LPS）谱分析、脉冲场凝胶电泳（PFGE）谱分析以及用REP和ERIC引物进行的rep-PCR（使用重复序列作为基因组指纹图谱的PCR引物）来研究非共生特性，而用质粒谱分析以及用nif基因探针、nodB、nodD、nod框和来自共生区域的IS序列作为探针进行杂交，以揭示共生基因间的关系。结果用于菌株间距离的成对计算，这些距离以树状图的形式直观呈现。对所有汇总试验以及分别对染色体试验和共生标记的关联指数进行比较，以展示不同类别试验对菌株分组的贡献。我们的研究表明，加氏根瘤菌中与共生相关的遗传特征将该物种的菌株分为两组，分别对应于与东方山羊豆和药用山羊豆形成有效共生的菌株。因此，我们建议将与东方山羊豆形成有效共生的加氏根瘤菌菌株称为加氏根瘤菌生物变种东方型，将与药用山羊豆形成有效共生的菌株称为加氏根瘤菌生物变种药用型。
