Ghazarossian V, Laney M, Vorpahl J, Pease J, Skold C, Watts R, Jeong H, Dafforn A, Cook R, Ullman E F
Syva Co., Research Department, Palo Alto, CA 94303.
Clin Chem. 1988 Sep;34(9):1720-5.
The system described here can distinguish between single and agglutinated erythrocytes by use of a non-flow fiber optic fluorometer. The method is capable of detecting cell-surface antigens and antibodies to cell-surface antigens present in blood. Significant features include high-efficiency fluorescent dyes that intercalate into cell membranes, a stretching membrane for transport and mixing of samples, charged colloidal magnetite for magnetic separation of erythrocytes, and an immersible fiber optic probe for measuring fluorescence associated with cells in a 1-nL volume of a bulk solution. We describe the application of the system to automation of ABO/Rh grouping and antibody screening.
本文所述系统可通过使用非流动光纤荧光计区分单个红细胞和凝集红细胞。该方法能够检测血液中存在的细胞表面抗原以及针对细胞表面抗原的抗体。其显著特点包括可嵌入细胞膜的高效荧光染料、用于样品运输和混合的拉伸膜、用于红细胞磁分离的带电胶体磁铁矿以及用于测量与1纳升大量溶液中的细胞相关荧光的可浸入式光纤探头。我们描述了该系统在ABO/Rh血型分组和抗体筛查自动化方面的应用。
