De Waele P, Feys V, Van de Voorde A, Molemans F, Fiers W
Laboratory of Molecular Biology, State University of Gent, Belgium.
Eur J Biochem. 1988 Sep 15;176(2):287-95. doi: 10.1111/j.1432-1033.1988.tb14280.x.
From a mouse hybridoma cell line secreting a monoclonal antibody directed against the tumour marker human placental alkaline phosphatase, mRNA coding for the H and L chains of this antibody was isolated and cloned as cDNA. Sequence analysis of the H and L chain cDNAs confirmed the IgG2b,kappa subtype previously established. Recloning the H and L chain cDNA information into SV40-based vectors enabled us to obtain expression of functional immunoglobulin upon cotransfection into COS or CHO dhfr- cells. This illustrates that non-lymphoid cells also have the capacity to assemble active immunoglobulins.
