Flamez D, Remaut E, Fiers W
Laboratory of Molecular Biology, Gent University, Belgium.
J Biotechnol. 1995 Sep 29;42(2):133-43. doi: 10.1016/0168-1656(95)00072-x.
We report the production in Escherichia coli of a murine antibody IgG2b, a murine::human chimeric antibody IgG3 and the corresponding F(ab')2 fragments, all directed against human placental alkaline phosphatase, a tumor marker. The cDNA of the heavy chain of the mature antibodies and their fragments were linked up to the bacterial alkaline phosphatase signal sequence and were placed under control of the inducible tac promoter. Coexpression with the murine kappa light chain resulted in production of functional dimeric, trimeric and tetrameric, mature antibodies and F(ab')2 fragments in the periplasm of E. coli in a yield of 200-300 micrograms l-1. High amounts of light and heavy chains were present also in the insoluble fraction.
我们报道了在大肠杆菌中生产鼠源抗体IgG2b、鼠源-人嵌合抗体IgG3以及相应的F(ab')2片段，所有这些都针对肿瘤标志物人胎盘碱性磷酸酶。成熟抗体及其片段重链的cDNA与细菌碱性磷酸酶信号序列相连，并置于可诱导的tac启动子控制之下。与鼠源κ轻链共表达导致在大肠杆菌周质中产生功能性二聚体、三聚体和四聚体成熟抗体以及F(ab')2片段，产量为200 - 300微克/升。不溶性部分中也存在大量的轻链和重链。
