Poltorak M, Freed W J
Preclinical Neurosciences Section, National Institute of Mental Health, Saint Elizabeths Hospital, Washington, D.C. 20032.
Exp Brain Res. 1988;71(1):163-70. doi: 10.1007/BF00247531.
Fragments of normal embryonic cerebellum were transplanted into adult Quaking mice to examine using peroxidase-antiperoxidase immunocytochemistry the development of genetically normal tissue in an abnormal host environment. The Quaking mouse animals used as hosts are characterized by defective myelin associated glycoprotein. Normal characteristic expression and distribution of neurofilaments was observed in the cerebellar grafts. Nonphosphorylated epitopes of neurofilaments were seen in Purkinje cell bodies and dendrites. The phosphorylated epitopes of neurofilaments were observed in basket cell axons. Phosphorylated epitopes were also present in numerous myelinated axons, which were probably fibers from deep cerebellar nuclei. These data support the notion that neurogenesis with alternative connections can occur in transplants. Staining patterns with myelin associated glycoprotein and myelin basic protein also suggest that normal myelination occurs in grafts transplanted to the brains of Quaking mice.
将正常胚胎小脑的片段移植到成年颤抖小鼠体内，以利用过氧化物酶-抗过氧化物酶免疫细胞化学方法，研究在异常宿主环境中基因正常组织的发育情况。用作宿主的颤抖小鼠动物的特征是髓鞘相关糖蛋白有缺陷。在小脑移植体中观察到神经丝的正常特征性表达和分布。在浦肯野细胞体和树突中可见神经丝的非磷酸化表位。在篮状细胞轴突中观察到神经丝的磷酸化表位。磷酸化表位也存在于许多有髓轴突中，这些轴突可能是来自小脑深部核团的纤维。这些数据支持这样一种观点，即移植中可以发生具有替代连接的神经发生。髓鞘相关糖蛋白和髓鞘碱性蛋白的染色模式也表明，移植到颤抖小鼠大脑中的移植体发生了正常的髓鞘形成。
