Cole G T, Seshan K R, Franco M, Bukownik E, Sun S H, Hearn V M
Department of Botany, University of Texas, Austin 78713-7640.
Infect Immun. 1988 Oct;56(10):2686-94. doi: 10.1128/iai.56.10.2686-2694.1988.
A previously undescribed, immunoreactive, membranous spherule outer wall (SOW) fraction produced by Coccidioides immitis (strains 634 and 735) grown in culture was isolated. Both this fraction and intact spherules were reactive with sera from coccidioidomycosis patients, as demonstrated by immunofluorescence microscopy. The serological activity of SOW was also demonstrated by its reactivity with human anti-C. immitis tube precipitin in a standardized immunodiffusion assay. Extraction of SOW with the nonionic detergent N-octyl-beta-D-glucopyranoside (OG) permitted the isolation of an OG-soluble fraction which was reactive in the immunodiffusion assay. Rabbit antisera raised against the OG-soluble fraction were used in immunofluorescence and immunoelectron-microscopic studies of the parasitic cycle to confirm that the immunoreactive components of the solubilized fraction of SOW were associated with the inner and outer layers of the spherule wall as well as with distinct cytoplasmic organelles observed in thin sections of spherules. The immunoreactivity of SOW with sera from patients suggested that infected individuals are exposed to this surface wall material isolated from in vitro-grown spherules.
从在培养基中生长的粗球孢子菌（菌株634和735）产生的一种先前未描述的、具有免疫反应性的膜状小球外壁（SOW）组分被分离出来。通过免疫荧光显微镜观察证实，该组分和完整的小球均与球孢子菌病患者的血清发生反应。在标准化免疫扩散试验中，SOW与人抗粗球孢子菌试管沉淀素的反应性也证明了其血清学活性。用非离子去污剂N-辛基-β-D-吡喃葡萄糖苷（OG）提取SOW可分离出在免疫扩散试验中有反应性的OG可溶级分。用针对OG可溶级分产生的兔抗血清进行寄生周期的免疫荧光和免疫电子显微镜研究，以确认SOW可溶级分的免疫反应性成分与小球壁的内层和外层以及在小球薄片中观察到的不同细胞质细胞器有关。SOW与患者血清的免疫反应性表明，受感染个体接触到了从体外培养的小球中分离出的这种表面壁物质。
