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粗球孢子菌的一种具有免疫反应性的水溶性分生孢子壁组分。

An immunoreactive, water-soluble conidial wall fraction of Coccidioides immitis.

作者信息

Cole G T, Kirkland T N, Sun S H

出版信息

Infect Immun. 1987 Mar;55(3):657-67. doi: 10.1128/iai.55.3.657-667.1987.

DOI:10.1128/iai.55.3.657-667.1987
PMID:3102379
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC260390/
Abstract

Arthroconidia stripped of their outer, hydrophobic wall layer release an immunoreactive, water-soluble fraction (SCWF), the composition of which is examined in this paper. The immunogenicity of SCWF was determined by its reactivity with anti-Coccidioides immitis complement-fixing antibody and tube precipitin antibody in a standardized immunodiffusion assay as well as reactivity in a lymphocyte proliferation assay. SCWF was shown to be more immunoreactive for immune lymphocytes than selected cytosol and culture supernatant fractions of C. immitis. Rabbit antisera raised against SCWF were used in immunoelectron-microscopic and immunofluorescence studies to confirm that the immunoreactive components of SCWF are primarily associated with the inner conidial wall. The antigenic composition of the conidial wall fraction was characterized by advancing-line immunoelectrophoresis with the previously established coccidioidin reference system. Protein composition was characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Chromatographic fractionation of SCWF was performed on a Sephacryl S-300 preparative column. Selected fractions which showed significantly higher immunoreactivity and less complex antigenic composition than whole SCWF were characterized. Two heat-sensitive antigens of Sephacryl fraction 3a were identified. One or both of these antigens may correspond to the complement-fixing antigen, which is potentially important as an immunodiagnostic antigen. Fraction 4 contained a previously described wall-associated antigen (AgCS) which may also be of immunodiagnostic value. We conclude that the conidial envelope is a reservoir of immunoreactive macromolecules which may play significant roles in early stages of infection.

摘要

去除外层疏水壁层的关节孢子会释放出一种具有免疫反应性的水溶性组分（SCWF），本文对其组成进行了研究。通过在标准化免疫扩散试验中SCWF与抗粗球孢子菌补体结合抗体和试管沉淀素抗体的反应性以及淋巴细胞增殖试验中的反应性来确定SCWF的免疫原性。结果表明，SCWF对免疫淋巴细胞的免疫反应性比粗球孢子菌的选定胞质溶胶和培养上清液组分更强。用针对SCWF产生的兔抗血清进行免疫电子显微镜和免疫荧光研究，以证实SCWF的免疫反应性成分主要与分生孢子内壁相关。通过用先前建立的球孢子菌素参考系统进行前进线免疫电泳来表征分生孢子壁组分的抗原组成。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳来表征蛋白质组成。在Sephacryl S-300制备柱上对SCWF进行色谱分离。对选定的组分进行了表征，这些组分显示出比整个SCWF更高的免疫反应性和更简单的抗原组成。鉴定出Sephacryl组分3a的两种热敏感抗原。这些抗原中的一种或两种可能对应于补体结合抗原，作为免疫诊断抗原可能具有重要意义。组分4含有一种先前描述的壁相关抗原（AgCS），其也可能具有免疫诊断价值。我们得出结论，分生孢子包膜是免疫反应性大分子的储存库，可能在感染早期发挥重要作用。

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