中药复方乐草石对乙型肝炎的影响：体内和体外研究。
Effects of traditional Chinese medicine formula Le-Cao-Shi on hepatitis B: In vivo and in vitro studies.
机构信息
Key Laboratory of Marine Drugs, The Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003, PR China; Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, PR China.
Key Laboratory of Marine Drugs, The Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003, PR China; Marine Science & Technology Institute, College of Environmental Science & Engineering, Yangzhou University, Yangzhou, 225127, PR China.
出版信息
J Ethnopharmacol. 2019 Nov 15;244:112132. doi: 10.1016/j.jep.2019.112132. Epub 2019 Aug 2.
ETHNOPHARMACOLOGICAL RELEVANCE
Formula Le-Cao-Shi (LCS) is a traditional Chinese medicine (TCM), which has long been used as a folk remedy against hepatitis B in China. The present study was conducted to evaluate the anti-hepatitis B effects of aqueous extract of LCS in vivo and in vitro.
MATERIALS AND METHOD
we investigated the anti-HBV effects of LCS in vivo and in vitro with duck hepatitis B model and HepG2.2.15 cell line model, respectively. The serologic and cellular biomarkers and the histopathological changes were examined.
RESULTS
By a duck hepatitis B model, the extract of LCS was found to restrain the expressions of duck hepatitis B surface antigen (DHBsAg), hepatitis B e antigen (DHBeAg), and HBV-DNA (DHBV-DNA). Moreover, LCS could decrease the levels of aspartate and alanine aminotransferases (AST and ALT) and ameliorate duck liver histological lesions. Correspondingly, in a HepG2.2.15 cellular model, LCS could also significantly inhibit the secretions of HBsAg and HBeAg.
CONCLUSION
LCS exerted potent anti-hepatitis effects against the infection of HBV. The above results demonstrated the first-hand experimental evidences for the anti-hepatitis B efficiency of LCS. Our study provides a basis for further exploration and development of this promising compound prescription to treat hepatitis B disease.
民族药理学相关性
配方 Le-Cao-Shi（LCS）是一种中药，长期以来在中国一直被用作治疗乙型肝炎的民间药物。本研究旨在评估 LCS 水提物在体内和体外抗乙型肝炎病毒的作用。
材料和方法
我们分别使用鸭乙型肝炎模型和 HepG2.2.15 细胞系模型研究了 LCS 对 HBV 的体内和体外抗作用。检查了血清和细胞生物标志物以及组织病理学变化。
结果
通过鸭乙型肝炎模型，发现 LCS 提取物可抑制乙型肝炎表面抗原（DHBsAg）、乙型肝炎 e 抗原（DHBeAg）和 HBV-DNA（DHBV-DNA）的表达。此外，LCS 还可以降低天冬氨酸转氨酶和丙氨酸转氨酶（AST 和 ALT）的水平，并改善鸭肝组织学病变。相应地，在 HepG2.2.15 细胞模型中，LCS 还可以显著抑制 HBsAg 和 HBeAg 的分泌。
结论
LCS 对 HBV 感染具有强烈的抗肝炎作用。上述结果为 LCS 的抗乙型肝炎 B 效率提供了第一手实验证据。我们的研究为进一步探索和开发这种有前途的复方处方治疗乙型肝炎疾病提供了依据。
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