新型胶原蛋白止血基质的评估：两种止血基质在兔空肠动脉损伤模型中的比较。

Evaluation of a Novel Collagen Hemostatic Matrix: Comparison of Two Hemostatic Matrices in a Rabbits Jejunal Artery Injury Model.

机构信息

Division of Endocrine Surgery, Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea.

出版信息

J Surg Res. 2019 Nov;243:553-559. doi: 10.1016/j.jss.2019.05.017. Epub 2019 Aug 2.

DOI:10.1016/j.jss.2019.05.017

PMID:31382076

Abstract

BACKGROUND

We evaluated the hemostatic efficacy and immunogenicity of CollaStat compared with FloSeal in a rabbit jejunal artery injury model.

METHODS

A total of 27 experimental rabbits were used in the study. For each hemostatic agent, an injury was created in one of the right angles of the jejunal arteries originating from the vascular arcs. Time to hemostasis was determined after applying manual compression to the wound for 30 s, which was repeated a maximum of three times in cases of persistent bleeding. On postoperative day 7, the concentration of serum antithrombin antibody was measured among agent-treated and nontreated control groups.

RESULTS

The mean time to hemostasis for CollaStat was significantly shorter than for FloSeal (64.0 ± 5.0 versus 84.0 ± 7.8 s; P = 0.040). There were no significant differences in rabbit serum mean anti-thrombin Ab concentration between CollaStat-treated, FloSeal -treated, and the control groups (8.43 ± 0.44 versus 8.18 ± 7.8 versus 9.58 ± 1.11 ng/mL; P = 0.065).

CONCLUSIONS

According to our study, CollaStat was more efficient in achieving hemostasis in a rabbit jejunal artery injury and exhibited nonsignificant immunogenicity compared with FloSeal. These findings suggest that CollaStat has acceptable hemostatic potential for controlling significant arterial bleeding.

摘要

背景

我们在兔空肠动脉损伤模型中评估了 CollaStat 与 FloSeal 的止血效果和免疫原性。

方法

本研究共使用 27 只实验兔。对于每种止血剂，在源自血管弓的空肠动脉的一个直角处造成损伤。在对伤口进行 30 秒的手动压迫后，确定止血时间，如果持续出血，则最多重复 3 次。在术后第 7 天，在接受治疗的和未接受治疗的对照组中测量血清抗凝血酶抗体的浓度。

结果

CollaStat 的平均止血时间明显短于 FloSeal（64.0±5.0 秒与 84.0±7.8 秒；P=0.040）。CollaStat 治疗组、FloSeal 治疗组和对照组的兔血清平均抗凝血酶 Ab 浓度无显著差异（8.43±0.44 纳克/毫升与 8.18±7.8 纳克/毫升与 9.58±1.11 纳克/毫升；P=0.065）。