Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, China.
Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, China.
Biosens Bioelectron. 2019 Oct 1;142:111551. doi: 10.1016/j.bios.2019.111551. Epub 2019 Jul 31.
This work reported a ternary quenching strategy based on electrochemiluminescence resonance energy transfer (ECL-RET) between luminol/HO system and MnO capped carbon nanospheres (MnO@C) and Mn released from the reduction of MnO and ascorbic acid (AA) consumed superoxide radical (O) for detection of insulin. To improve the ECL behavior of luminol, AuPdPt hybrid nanoparticles decorated MoS nanosheet-coated TiO nanobelt (AuPdPt-MoS@TiO) were applied to combine with luminol and primary antibodies via covalent linkage which exhibited perfectly catalysis for the electroreduction of HO. In order to sensitively detect insulin, labels MnO@C were used to anchor secondary antibodies via electrostatic interaction and inhibit the ECL intensity of luminol. The perfect overlap of the ECL spectrum of luminol with the absorption spectrum of MnO allowed the ECL-RET to decrease ECL signals of luminol. Specifically, AA was introduced not only to inhibit the ECL of luminol, but also to reduce MnO producing Mn which can be reacted with O to further quench the ECL reaction between luminol and HO. On the basis of the dual quenching effect of luminol, the sandwich immunosensor was prepared to determine insulin with wide linear range from 0.5 pg mL to 60 ng mL and a low detection limit of 0.13 pg mL (S/N = 3).
这项工作报道了一种基于电化学发光共振能量转移（ECL-RET）的三元猝灭策略，该策略基于鲁米诺/HO 体系与 MnO 封端的碳纳米球（MnO@C）之间的相互作用，以及 MnO 被还原为 Mn 和抗坏血酸（AA）消耗超氧自由基（O）用于检测胰岛素。为了提高鲁米诺的 ECL 行为，应用了 AuPdPt 杂化纳米粒子修饰的 MoS 纳米片涂层 TiO 纳米带（AuPdPt-MoS@TiO）与鲁米诺和一抗通过共价键结合，从而对 HO 的电化学还原表现出完美的催化作用。为了灵敏地检测胰岛素，MnO@C 通过静电相互作用被用于固定二抗，并抑制鲁米诺的 ECL 强度。鲁米诺的 ECL 光谱与 MnO 的吸收光谱的完美重叠允许 ECL-RET 降低鲁米诺的 ECL 信号。具体而言，AA 的引入不仅抑制了鲁米诺的 ECL，而且还还原了 MnO 生成 Mn，Mn 可以与 O 反应进一步猝灭鲁米诺和 HO 之间的 ECL 反应。基于鲁米诺的双重猝灭效应，制备了夹心型免疫传感器用于检测胰岛素，线性范围从 0.5 pg/mL 到 60 ng/mL，检测限低至 0.13 pg/mL（S/N=3）。
