Hormia M, Kariniemi A L, Laitinen L, Virtanen I
Department of Pathology, University of Helsinki, Finland.
J Histochem Cytochem. 1988 Oct;36(10):1231-7. doi: 10.1177/36.10.3138306.
Dolichos biflorus agglutinin (DBA) binds to N-acetyl-D-galactosamine (GalNAc) residues in glycoconjugates and agglutinates erythrocytes carrying blood group antigen A. In cryostat sections of various tissues from blood group-specified humans, fluorochrome-coupled DBA bound preferentially to fusiform connective tissue cells and to certain epithelial cells. The connective tissue cells were identified as mast cells by their typical metachromasia in consecutive staining with toluidine blue. Double labeling with DBA and conjugated avidin revealed two distinct populations of mast cells. In several tissues the DBA-reactive cells likewise displayed uniform avidin reactivity. In intestinal mucosa, however, morphologically distinct DBA-binding mast cells were found, which were labeled with the avidin conjugates only in specially fixed paraffin sections. DBA did not bind to vascular endothelial cells, which could be identified by double staining with antibodies to factor VIII-related antigen. Labeling with Helix pomatia agglutinin (HPA), another blood group A-reactive lectin, resulted in distinct blood group-dependent fluorescence of the endothelia. Sophora japonica agglutinin (SJA), a blood group B-reactive lectin, labeled vascular endothelial cells in tissues from blood group A, AB, and B donors. HPA and SJA reacted with small mast cells in the gastrointestinal mucosa but failed to label large mast cells in any of the tissues. These results indicate that the blood group reactivity of lectins, as determined by erythroagglutination, is not necessarily consistent with their reactivity with blood group determinants in tissue sections. Moreover, DBA conjugates appear to be a reliable probe for detection of mast cells in various human connective tissues.
双花扁豆凝集素（DBA）可与糖缀合物中的N-乙酰-D-半乳糖胺（GalNAc）残基结合，并凝集携带血型A抗原的红细胞。在血型明确的人类各种组织的冰冻切片中，荧光染料偶联的DBA优先与梭形结缔组织细胞及某些上皮细胞结合。通过甲苯胺蓝连续染色时典型的异染性，结缔组织细胞被鉴定为肥大细胞。用DBA和共轭抗生物素蛋白进行双重标记显示出两种不同类型的肥大细胞。在几种组织中，与DBA反应的细胞同样表现出一致的抗生物素蛋白反应性。然而，在肠黏膜中，发现了形态上不同的与DBA结合的肥大细胞，这些细胞仅在特殊固定的石蜡切片中被抗生物素蛋白共轭物标记。DBA不与血管内皮细胞结合，血管内皮细胞可通过与VIII因子相关抗原抗体的双重染色来鉴定。用另一种与血型A反应的凝集素——蜗牛凝集素（HPA）标记，导致内皮细胞出现明显的血型依赖性荧光。血型B反应性凝集素——槐凝集素（SJA）标记了来自血型A、AB和B供体组织中的血管内皮细胞。HPA和SJA与胃肠道黏膜中的小肥大细胞反应，但未能标记任何组织中的大肥大细胞。这些结果表明，通过红细胞凝集测定的凝集素血型反应性不一定与其在组织切片中与血型决定簇的反应性一致。此外，DBA共轭物似乎是检测各种人类结缔组织中肥大细胞的可靠探针。
