Nakazato T, Akiyama A
Department of Neurology, Juntendo University School of Medicine, Tokyo, Japan.
J Neurochem. 1988 Oct;51(4):1007-13. doi: 10.1111/j.1471-4159.1988.tb03061.x.
Freely moving rats were injected intraventricularly with 200 micrograms of 6-hydroxydopamine (6-OHDA) and examined for transport of this substance to the striatum, degradation of dopamine (DA) neurons, and functional recovery through microcomputer-controlled in vivo voltammetry. Approximately 6 min after the injection, 6-OHDA began to appear in the extracellular fluid of the contralateral striatum. It increased linearly and began to decrease exponentially with the termination of the injection. Two hours after the injection with 6-OHDA, a peak began to appear at the same potential as the peak of DA in the differential pulse voltammogram. It persisted for approximately 3 days. When the rats were injected intraperitoneally with L-3,4-dihydroxyphenylalanine (L-Dopa), the conversion of L-Dopa to DA was not found 1 week after the 6-OHDA injection, but was clearly recognized 5 months after the injection. When the rats were examined for behavioral changes arising from the L-Dopa injection, they were found to be clearly less hyperactive 5 months after the 6-OHDA injection than 1 week after.
对自由活动的大鼠脑室内注射200微克6-羟基多巴胺（6-OHDA），并通过微机控制的体内伏安法检测该物质向纹状体的转运、多巴胺（DA）神经元的降解以及功能恢复情况。注射后约6分钟，6-OHDA开始出现在对侧纹状体的细胞外液中。它呈线性增加，并在注射结束时开始呈指数下降。注射6-OHDA两小时后，在微分脉冲伏安图中与DA峰相同的电位处开始出现一个峰。该峰持续约3天。当给大鼠腹腔注射L-3,4-二羟基苯丙氨酸（L-Dopa）时，在6-OHDA注射1周后未发现L-Dopa转化为DA，但在注射5个月后可明显观察到。当检测大鼠因注射L-Dopa引起的行为变化时，发现6-OHDA注射5个月后的大鼠比注射1周后的大鼠明显不那么多动。
