Universitat de Lleida (UdL), Lleida, Spain.
Institut de Recerca Biomèdica de Lleida (IRB-Lleida), Lleida, Spain.
Sci Rep. 2019 Aug 5;9(1):11313. doi: 10.1038/s41598-019-47906-x.
Multiple sclerosis (MS) is a complex multifactorial neuropathology. Although its etiology remains unclear, it has been demonstrated that the immune system attacks myelin, leading to demyelination and axonal damage. The involvement of lipids as one of the main components of myelin sheaths in MS and other demyelinating diseases has been postulated. However, it is still a matter of debate whether specific alteration patterns exist over the disease course. Here, using a lipidomic approach, we demonstrated that, at the time of diagnosis, the cerebrospinal fluid of MS patients presented differences in 155 lipid species, 47 of which were identified. An initial hierarchical clusterization was used to classify MS patients based on the presence of 25 lipids. When a supervised method was applied in order to refine this classification, a lipidomic signature was obtained. This signature was composed of 15 molecules belonging to five different lipid families including fatty acids (FAs). An FA-targeted approach revealed differences in two members of this family: 18:3n3 and 20:0 (arachidic acid). These results reveal a CSF lipidomic signature in MS patients at the time of diagnosis that might be considered as a potential diagnostic tool.
多发性硬化症（MS）是一种复杂的多因素神经病理学。尽管其病因仍不清楚，但已经证明免疫系统攻击髓鞘，导致脱髓鞘和轴突损伤。脂质作为髓鞘的主要成分之一参与多发性硬化症和其他脱髓鞘疾病已经被假设。然而，在疾病过程中是否存在特定的改变模式仍存在争议。在这里，我们使用脂质组学方法表明，在诊断时，MS 患者的脑脊液中存在 155 种脂质种类的差异，其中 47 种已被鉴定。最初使用层次聚类法根据 25 种脂质的存在来对 MS 患者进行分类。当应用监督方法来完善这种分类时，获得了一个脂质组学特征。该特征由属于五个不同脂质家族的 15 种分子组成，包括脂肪酸（FAs）。FA 靶向方法揭示了该家族中两个成员的差异：18:3n3 和 20:0（花生四烯酸）。这些结果揭示了 MS 患者在诊断时的脑脊液脂质组学特征，可被视为一种潜在的诊断工具。
