Jové Mariona, Pradas Irene, Naudí Alba, Rovira-Llopis Susana, Bañuls Celia, Rocha Milagros, Portero-Otin Manuel, Hernández-Mijares Antonio, Victor Victor M, Pamplona Reinald
Department of Experimental Medicine, Lleida University-Institute for Research in Biomedicine of Lleida (UdL-IRBLleida), 25198 Lleida, Spain.
Foundation for the Promotion of Healthcare and Biomedical Research in the Valencian Community (FISABIO), Service of Endocrinology, University Hospital Dr. Peset, 46017 Valencia, Spain.
Oncotarget. 2017 Dec 17;9(4):4522-4536. doi: 10.18632/oncotarget.23393. eCollection 2018 Jan 12.
In this work, a non-targeted approach was used to unravel changes in the plasma lipidome of PCOS patients. The aim is to offer new insights in PCOS patients strictly selected in order to avoid confounding factors such as dyslipemia, obesity, altered glucose/insulin metabolism, cardiovascular disease, or cancer.
Multivariate statistics revealed a specific lipidomic signature for PCOS patients without associated pathologies. This signature implies changes, mainly by down-regulation, in glycerolipid, glycerophospholipid and sphingolipid metabolism suggesting an altered biosynthetic pathway of glycerophospholipids and cell signaling as second messengers in women with PCOS.
Our study confirms that a lipidomic approach discriminates a specific phenotype from PCOS women without associated pathologies from healthy controls.
In a cross-sectional pilot study, data were obtained from 34 subjects, allocated to one of two groups: a) lean, healthy controls ( = 20), b) PCOS patients ( = 14) with diagnosis based on hyperandrogenaemia, oligo-anovulation and abnormal ovaries with small follicular cysts. A detailed biochemical characterization was made and lipidomic profiling was performed via an untargeted approach using LC-ESI-QTOF MS/MS.
在本研究中，采用非靶向方法来揭示多囊卵巢综合征（PCOS）患者血浆脂质组的变化。目的是为经过严格挑选以避免诸如血脂异常、肥胖、糖/胰岛素代谢改变、心血管疾病或癌症等混杂因素的PCOS患者提供新的见解。
多变量统计揭示了无相关病理状况的PCOS患者的特定脂质组特征。该特征表明甘油脂、甘油磷脂和鞘脂代谢发生了变化，主要是下调，这表明PCOS女性中甘油磷脂的生物合成途径和作为第二信使的细胞信号传导发生了改变。
我们的研究证实，脂质组学方法能够区分无相关病理状况的PCOS女性与健康对照的特定表型。
在一项横断面试点研究中，从34名受试者获取数据，这些受试者被分为两组之一：a）瘦的健康对照组（n = 20），b）基于高雄激素血症、少排卵和伴有小卵泡囊肿的异常卵巢诊断的PCOS患者（n = 14）。进行了详细的生化特征分析，并通过使用LC-ESI-QTOF MS/MS的非靶向方法进行脂质组分析。
