Pfaller M A, Riley J, Gerarden T
Veterans Administration Medical Center, Iowa City, Iowa 52240.
J Med Vet Mycol. 1988 Apr;26(2):119-26.
The ability of two known inhibitors of polyamine synthesis, alpha-difluoromethylornithine (DFMO), an inhibitor of ornithine decarboxylase (ODC), and cyclohexylamine, an inhibitor of spermidine synthase, to inhibit the in vitro growth and polyamine synthesis of clinical isolates of Candida tropicalis and Candida albicans was examined. Treatment of C. tropicalis and C. albicans with either DFMO or cyclohexylamine resulted in depletion of cellular polyamines and inhibition of growth. The growth inhibition produced by each of these compounds was completely reversed by exogenous polyamines. Depletion of polyamines by low concentrations of DFMO significantly enhanced the growth inhibitory activity of cyclohexylamine versus C. albicans. DFMO inhibited ODC activity in both C. albicans and C. tropicalis. These findings document the ability of cyclohexylamine and DFMO to inhibit polyamine synthesis and growth in clinically important species of Candida.
研究了两种已知的多胺合成抑制剂，即鸟氨酸脱羧酶（ODC）抑制剂α-二氟甲基鸟氨酸（DFMO）和亚精胺合酶抑制剂环己胺，对热带念珠菌和白色念珠菌临床分离株体外生长和多胺合成的抑制能力。用DFMO或环己胺处理热带念珠菌和白色念珠菌会导致细胞内多胺耗尽并抑制生长。这些化合物各自产生的生长抑制作用可被外源性多胺完全逆转。低浓度DFMO使多胺耗尽显著增强了环己胺对白色念珠菌的生长抑制活性。DFMO抑制白色念珠菌和热带念珠菌中的ODC活性。这些发现证明了环己胺和DFMO抑制临床上重要念珠菌属物种多胺合成和生长的能力。
