Moschonas N K, Thireos G, Kafatos F C
Institute of Molecular Biology and Biotechnology, University of Crete, Greece.
J Mol Evol. 1988;27(3):187-93. doi: 10.1007/BF02100073.
We report a preliminary analysis of structural and regulatory evolution of the A and B chorion gene families in two wild silkmoths, Antheraea pernyi and Antheraea polyphemus. Homospecific and heterospecific dot hybridizations were performed between previously characterized A. polyphemus complementary DNA clones and total or stage-specific follicular mRNAs from the two species. The hybridization patterns indicated substantial interspecies changes in the abundance of corresponding mRNA sequences (heteroposic evolution) without substantial changes in their developmental specificities (heterochronic evolution). In addition, the proteins encoded in the two species by corresponding mRNAs were determined by hybrid-selected translation followed by electrophoretic analysis. The results suggested that the proteins evolve in size, presumably through internal deletions and duplications.
我们报告了对两种野生蚕蛾，即柞蚕和多音天蚕中A和B绒毛膜基因家族的结构和调控进化的初步分析。使用先前鉴定的多音天蚕互补DNA克隆与这两个物种的总卵泡mRNA或阶段特异性卵泡mRNA进行了同种和异种斑点杂交。杂交模式表明，相应mRNA序列的丰度存在显著的种间变化（异位进化），而其发育特异性没有显著变化（异时进化）。此外，通过杂交选择翻译后进行电泳分析，确定了两个物种中由相应mRNA编码的蛋白质。结果表明，这些蛋白质在大小上发生了进化，可能是通过内部缺失和重复实现的。
