一个编码已知A家族绒毛膜蛋白的cDNA克隆的筛选与序列分析。
Selection and sequence analysis of a cDNA clone encoding a known chorion protein of the A family.
作者信息
Tsitilou S G, Regier J C, Kafatos F C
出版信息
Nucleic Acids Res. 1980 May 10;8(9):1987-97. doi: 10.1093/nar/8.9.1987.
Abstract
Using as criteria the size, abundance and developmental specificity of hybridizing mRNA sequences, we have selected from our chorion cDNA library a clone corresponding to a specific chorion protein, A4--cl. Comparison between the clone sequence and the largely known sequence of A4--cl validates the use of the cDNA library for sequence analysis of the chorion multigene families. The two major chorion protein families, A and B, share certain structural similarities.
摘要
以杂交mRNA序列的大小、丰度和发育特异性为标准，我们从绒毛膜cDNA文库中筛选出了一个与特定绒毛膜蛋白A4-cl相对应的克隆。该克隆序列与已知的A4-cl序列进行比较，证实了cDNA文库可用于绒毛膜多基因家族的序列分析。两个主要的绒毛膜蛋白家族A和B具有一定的结构相似性。
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